Journal of East China Normal University(Natural Sc ›› 2014, Vol. 2014 ›› Issue (4): 102-112.

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De novo transcriptome analysis and the phylogenetic position of glass lizards

ZHU Jing-jing1, LI Wan-shun2, GAO Hong1,  XU Tong2,  BAO Qiu-ying1, ZHENG Yong-xiang3,  ZHOU De-min3,  XIA Gang1   

  1. 1. Institutes for Advanced Interdisciplinary Research, East China Normal University, Shanghai 200062, China; 2. BGI-tech, Shenzhen Guangdong 518083, China; 3. State Key Laboratory of Natural and Biomimetic Drugs, Peaking University Health Science Center, Peaking University, Beijing 100191, China
  • Received:2013-10-01 Revised:2014-01-01 Online:2014-07-25 Published:2014-07-25

Abstract: Glass lizards are a group of reptiles that resemble snake
and possess many lizard's characteristics that contributed to its
ambiguous taxonomy. Meanwhile, glass lizards were recorded to have
many therapeutic applications in TCM. Hence, we present the
sequencing, de novo assembly and annotation of transcriptome from
\textit{Ophisaurus}\textit{ harti}'s gastrointestinal tract. A total
4.6~Gbp of high quality data was generated, 58~959 unigenes were
assembled and 35~708 (60.56{\%}) unigenes were annotated to the
public databases. To understand the evolutionary relationship among
glass lizard, snake and lizard, ortholog gene families and
phylogenic tree were performed, and the results all showed that
\textit{Ophisaurus}\textit{harti} is more closely related to the
snakes than to the lizard. A total of 10~613 cSSR markers from the
\textit{Ophisaurus}\textit{harti} transcriptome were identified and
1~644 markers were obtained using at least one primer with a strict
criterion. This is the first time to give insight into the
transcriptome and phylogenetic evolution in \textit{Ophisaurus
harti}. These sequences and markers will provide valuable sources
for \textit{Ophisaurus harti} studies.

Key words: glass lizard, Ophisaurus harti, phylogenetic evolution, transcriptome, cSSR

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