华东师范大学学报(自然科学版) ›› 2008, Vol. 2008 ›› Issue (2): 85-91.

• 生命科学 • 上一篇    下一篇

膨叶唇鳞苔原丝体发育及油体的观察

于传梅，郑敏，朱瑞良   

  1. 华东师范大学 生命科学学院, 上海200062
  • 收稿日期:2007-03-09 修回日期:2007-04-23 出版日期:2008-03-25 发布日期:2008-03-25
  • 通讯作者: 朱瑞良

Protonema development and observation on oil bodies of Cheilolejeunea ventricosa (Schiffn.) X.L.He(Chinese)

YU Chuan-mei，ZHENG Min，ZHU Rui-liang   

  1. School of Life Science, East China Normal University, Shanghai200062, China
  • Received:2007-03-09 Revised:2007-04-23 Online:2008-03-25 Published:2008-03-25
  • Contact: ZHU Rui-liang

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摘要： 首次研究了膨叶唇鳞苔（新拟）(Cheilolejeunea ventricosa)的孢子萌发、原丝体和油体发育及配子体发生的过程，结果表明：(1) 使用经过改良的MS培养基（不加氨基酸和维生素，pH值为6.2，琼脂和蔗糖浓度均为1%）能在55 d左右的时间利用孢子培养获得植物体；(2) 用0.1%的次氯酸钠溶液，消毒5 s是消毒孢子的最佳方案；(3) 油体在孢子萌发初期为均一型，到幼叶发生后，成为可见的聚合型，但聚合的程度比野生材料要弱.膨叶唇鳞苔的孢子萌发类型属于细鳞苔型（Lejeunea type）.

关键词: 膨叶唇鳞苔, 苔类植物, 油体, 孢子, 组织培养, 孢子萌发, 膨叶唇鳞苔, 苔类植物, 油体, 孢子, 组织培养, 孢子萌发

Abstract: The spores of Cheilolejeunea ventricosa (Schiffn.) X.L.He were cultured on MS media (without acid and vitamin, pH=6.2, with 1% agar and 1% sucrose). The sporeling development and oil body ontogeny were observed. After a 55-day-long axenic culture, young plants were obtained successfully. Sodium hypochlorite solution was used to sterilize the capsules. Our results revealed that the sterilizing treatment with 0.1% Sodium hypochlorite solution for a period of 5 seconds was optimal. Oil bodies in primary protonemata are nearly homogeneous, but distinctly compound in the leaf cells of the primary leafy shoots. The spore germination and sporeling of Cheilolejeunea ventricosa belongs to Lejeunea type.

Key words: liverworts, oil body, spores, tissue culture, sporelin, Cheilolejeunea ventricosa, liverworts, oil body, spores, tissue culture, sporelin

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