华东师范大学学报(自然科学版) ›› 2005, Vol. 2005 ›› Issue (3): 48-52.

• 化学 • 上一篇    下一篇

Au@SiO2修饰电极的制备及对Cyt c的直接电化学研究

周宇艳1,2 ,鲜跃仲1,2,刘芳1,2,胡艺1,2,周丽绘3,金利通1   

  1. 1.华东师范大学 化学系，上海200062;2.湖南大学 化学生物传感与计量学国家重点实验室，长沙410082;3.华东理工大学 分析测试中心，上海200237
  • 收稿日期:2004-09-15 修回日期:2004-11-12 出版日期:2005-08-25 发布日期:2005-08-25
  • 通讯作者: 金利通

Preparation of the Au@SiO2 Modified Electrode and Its Application to the Direct Electrochemistry of Cytochrome C(Chinese)

ZHOU Yu-yan 1,2,XIAN Yue-zhong 1,2,LIU Fang 1,2,HU Yi 1,2,ZHOU Li-hui3,JIN Li-tong 1   

  1. 1.Department of Chemistry, East China Normal University, Shanghai200062,China;2.State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University,Changsha410082, China;3.Center of Analysis and Research, East China University of Science and Technology,Shanghai2000237,China
  • Received:2004-09-15 Revised:2004-11-12 Online:2005-08-25 Published:2005-08-25
  • Contact: JIN Li-tong

摘要： 研究Au@SiO2复合纳米材料修饰电极的制备方法及其对细胞色素c (Cyt c)的电催化行为.Cyt c在该修饰电极上表现出一对氧化还原峰，且在1.0×10-7~2.0×10-5 mol/L浓度范围内，Cyt c峰电流与浓度呈良好的线性关系，检测下限为5.0×10-8 mol/L (S/N=3).还应用紫外可见光谱考察了Au@SiO2复合纳米材料的生物兼容性，结果表明，Au@SiO2材料为Cyt c提供了一个“近水”的微环境，使其在该修饰电极表面不易变性，因而具有良好的生物相容性.

关键词: Cyt c, Au@SiO2, 复合纳米材料, Cyt c, Au@SiO2, 复合纳米材料

Abstract: Au@SiO2 composite nanoparticles were prepared. By controlling synthesis conditions, the thickness of the silica shell could be varied in the range of tens to several hundred nanometers. Coupled with chitosan (CHIT), Au@SiO2/CHIT/GC modified electrode was fabricated. The electrode was applied to the determination of cytochrome c (Cyt c). The results of the experiments indicated that the currents of Cyt c were linear to its concentrations over the range from 1.0×10-7 to 2.0×10-5 mol/L and the calculated detection limits (S/N=3) was 5.0×10-8 mol/L. Further work could be done to apply this electrochemical method to preparing biosensor and detecting biologic samples.

Key words: Au@SiO2, nano composite material, cytochrome c, Au@SiO2, nano composite material

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