华东师范大学学报(自然科学版) ›› 2006, Vol. 2006 ›› Issue (2): 88-92.

• 生命科学 • 上一篇    下一篇

利用固定化微生物细胞羟基化左旋乙基甾烯双酮

李 骏, 耿 旭, 张佩君, 吴自荣   

  1. 华东师范大学 生命科学学院，上海 200062
  • 收稿日期:2004-12-29 修回日期:2005-04-13 出版日期:2006-03-25 发布日期:2006-03-25
  • 通讯作者: 吴自荣

15α-Hydroxylation of 13-ethy1-estr-4-ene-3,17-dione Using Immobilized Penicillium raistrickii by Calcium Alginate gel Embedding Methd(Chinese)

LI Jun, GENG Xu, ZHANG Pei-jun, WU Zi-rong   

  1. Shool of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2004-12-29 Revised:2005-04-13 Online:2006-03-25 Published:2006-03-25
  • Contact: WU Zi-rong

摘要：

研究了海藻酸钙包埋法固定化技术在甾体化合物的生物转化中的应用.利用固定化 Penicillium raistrickii 细胞实现对左旋乙基甾烯双酮（13-ethyl-estr-4-ene-3,17-dione）的15α 位羟基化作用，合成了重要的药物中间体15α-羟基左旋乙基甾烯双酮.优化了固定化孢子浓度，固定化孢子接种量，投料浓度，投料时间，转化时间等转化条件.结果表明：在摇瓶发酵生物转化中，固定化 Penicillium raistrickii 细胞可重复利用5次而转化能力没有明显下降.

关键词: Penicillium raistrickii, 15α 羟化, 固定化技术, Penicillium raistrickii, 15α 羟化, 固定化技术

Abstract:

15α-hydroxylation of 13-ethyl-estr-4-ene-3,17-dione using immobilized Penicillium raistrickii entrapped in calcium alginate gel was studied. The conversion conditions, which affected hydroxylation such as substrate concentration, the time to add substrate, conversion duration, were investigated. The results show that, the immobilized Penicillium raistrickii can be utilized repeatedly five times with no obvious decline of hydroxylation ability.

Key words: 15α-hydroxylation, immobilized method, Penicillium raistrickii, 15α-hydroxylation, immobilized method

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