华东师范大学学报(自然科学版) ›› 2008, Vol. 2008 ›› Issue (6): 133-136.

• 生命科学 • 上一篇    

纤毛虫胞质微管及大核的FLUTAX-EB荧光标记方法

李艺松，刘兰侠，史磊，顾福康   

  1. 华东师范大学 生命科学学院, 上海200062
  • 收稿日期:2008-05-09 修回日期:2008-08-25 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 顾福康

An improved FLUTAX-EB fluorenscent labeling method for visualizing microtubule and nucleolus of ciliate(Chinese)

LI Yi-song, LIU Lan-xia, SHI Lei, GU Fu-kang   

  1. School of Life Science, East China Normol University, Shanghai 200062, China
  • Received:2008-05-09 Revised:2008-08-25 Online:2008-11-25 Published:2008-11-25
  • Contact: GU Fu-kang

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摘要： 以游仆虫、尖毛虫和尾柱虫三种腹毛目纤毛虫为材料，在应用FLUTAX方法显示纤毛虫细胞微管结构的标本制备过程中，增加了EB染色的歩骤，使标记纤毛虫细胞皮层微管的同时也显示了细胞核的结构.并提出了实验中对EB浓度及渗透时间等需注意的方面.

关键词: 纤毛虫, 皮层, FLUTAX-EB荧光标记, 方法改进, 纤毛虫, 皮层, FLUTAX-EB荧光标记, 方法改进

Abstract: An employment of EB in FLUTAX fluorescent labeling porcedure was proposed, with the aim of visualizing both microtubular pattern and nucleolus of three species of ciliate including Euplotes woodruffi, Oxytricha sp. and Urostyla sp.. The concentration of EB, as well as the infiltrating time was also discussed.

Key words: cortex, FLUTAX-EB fluorenscent labeling, improved method, ciliate, cortex, FLUTAX-EB fluorenscent labeling, improved method

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