华东师范大学学报(自然科学版) ›› 2008, Vol. 2008 ›› Issue (6): 103-109.

• 生命科学 • 上一篇    下一篇

崇明水仙（Narcissus tazetta var. chinensis）不同外植体分化能力与鳞茎增大初探

熊莉君,李小方,王洋,孙越,许玲   

  1. 华东师范大学 生命科学学院，上海 200062
  • 收稿日期:2008-02-17 修回日期:2008-06-15 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 李小方

Differentiation of different explants and enlargement of tissue culture bulb of narcissus at Chongming (Narcissus tazetta var. chinensis) (Chinese)

XIONG Li-jun,LI Xiao-fang,WANG Yang,SUN Yue,XU Ling   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2008-02-17 Revised:2008-06-15 Online:2008-11-25 Published:2008-11-25
  • Contact: LI Xiao-fang

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摘要： 以崇明水仙鳞茎的上、中、下部、嫩叶和幼嫩花序为外植体，研究其在离体培养系统中的再分化能力，结果表明,带鳞茎盘的鳞茎和幼嫩花序最容易分化出小鳞茎.以组培获得的带鳞茎盘的鳞茎为材料建立快繁体系，40 d内小鳞茎数目增加3倍；并发现浓度适合的KH2PO4及暗培养能促进小鳞茎增大.组织学研究结果显示，组培水仙与大田生长水仙的顶端分生组织的形态结构不同，前者细胞分层不明显，细胞核大且染色较深.

关键词: 崇明水仙, 外植体, 分化能力, 鳞茎增大, 崇明水仙, 外植体, 分化能力, 鳞茎增大

Abstract: This paper studied the de-differentiation and differentiation of various explants such as bulb, leaf and infancy inflorescence of narcissus (Narcissus tazetta var. chinensis). The results showed that infrastructure and infancy inflorescence of narcissus were the best explants. When the infrastructure of adventitious buds was used as explants, the quantity of buds increased three times in forty days. Additionally, it was found that KH2PO4 of certain concentration and dark conditions were favorable to enlargement adventitious buds. The anatomic structure of shoot apical meristem of bulb from field was different from that of tissue culture bulb, which might be the reason why tissue culture bulbs were very hard to enlarge.

Key words: explants, differentiation, bulb enlargement, narcissus (Narcissus tazetta var. chinensis), explants, differentiation, bulb enlargement

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