华东师范大学学报(自然科学版) ›› 20120, Vol. 2012 ›› Issue (6): 81-88.doi:

• 生命科学 • 上一篇    下一篇

Mg2+对Bloom综合症解旋酶与G4DNA结合的影响研究

骆 衡1-3, 蔡明娟1,2, 陈 祥1, 2, 丁 玫1, 2, 李 坤1,2, 许厚强1,2   

  1. 1.贵州大学 动物科学学院 高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳 550025; 2.贵州大学 动物科学学院 贵州省动物遗传育种与繁殖重点实验室,贵阳 550025;
    3.贵州大学 农学院 贵州山地农业病虫害重点实验室,贵阳 550025
  • 收稿日期:2011-09-01 修回日期:2011-12-01 出版日期:2012-11-25 发布日期:2013-01-11

Study on the effects of Mg2+ on the binding between Bloom syndrome helicase and G4DNA

LUO Heng 1-3, CAI Ming-juan 1,2, CHEN Xiang 1,2, DING Mei 1,2,LI Kun 1,2, XU Hou-qiang 1,2   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Guizhou Key Laboratory of Animal Genetics, Breeding Reproduction, College of Animal Science, Guizhou University, Guiyang 550025, China; 3. The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Agricultural College, Guizhou University, Guiyang 550025, China
  • Received:2011-09-01 Revised:2011-12-01 Online:2012-11-25 Published:2013-01-11

摘要: 利用荧光偏振技术检测了Mg2+对G4DNA、BLM-G4DNA复合物和BLM642-1290解旋酶与G4DNA结合的影响.结果表明,G4DNA荧光偏振值随着Mg2+浓度的增加而增加(〖WTBX〗P〖WTBZ〗<0.01);BLM-G4DNA复合物的荧光偏振值随着Mg2+浓度的增加出现下降—升高—下降的变化趋势(〖WTBX〗P〖WTBZ〗<0.01);G4DNA与BLM642-1290解旋酶结合的荧光偏振值随着Mg2+浓度的增加而逐渐下降(〖WTBX〗P〖WTBZ〗<0.01);分析不同Mg2+浓度下两种分子结合的〖WTBX〗Kd〖WTBZ〗值,发现Mg2+浓度为3.0 mmol/L时,BLM642-1290解旋酶与G4DNA最容易结合,表明适量Mg2+浓度会促进BLM642-1290与G4DNA的结合,但会引起两种分子结合的形状、流动性和电荷等性质的改变.这些结果可为进一步研究BLM解旋酶对G4DNA的作用机理提供相关资料.

关键词: BLM解旋酶, G4DNA, Mg2+, 荧光偏振技术

Abstract: This paper studied the effects of Mg2+ on G4DNA, BLM-G4DNA complex, and the binding of BLM642-1290 helicase and G4DNA using fluorescence anisotropy technology.The results indicated that the fluorescence anisotropy of G4DNA increased with Mg2+ concentration (〖WTBX〗P〖WTBZ〗<0.01); The anisotropy of BLM-G4DNA complex showed varied tendency of decrease-increase-decrease with Mg2+ concentration (〖WTBX〗P〖WTBZ〗<0.01);The anisotropy of the binding between the helicase and G4DNA gradually declined following Mg2+ concentration (〖WTBX〗P〖WTBZ〗<0.01); The binding was most likely to occur between the helicase and G4DNA when Mg2+ concentrations was 3.0 mmol/L, which suggested that the suitable amount of Mg2+ could promote the binding of the helicase and G4DNA but that could lead to the change of the binding properties of two molecules including the shape, flow ability, and charge. These results provided useful data for studying the interaction mechanism between BLM helicase and G4DNA.

Key words: BLM helicase, G4DNA, Mg2+, fluorescence polarization technology

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