华东师范大学学报(自然科学版) ›› 20120, Vol. 2012 ›› Issue (6): 89-95.doi:

• 生命科学 • 上一篇    下一篇

REGγp53,NOTCH1BRAF在甲状腺癌中的表达及其临床意义

亓 龙1, 左 娣2, 王 辉1, 李晓涛2   

  1. 1.上海交通大学医学院附属新华医院 核医学科,上海 200092; 2.华东师范大学 生命医学研究所,上海 200241
  • 收稿日期:2012-01-01 修回日期:2012-04-01 出版日期:2012-11-25 发布日期:2013-01-11

Expressions of REGγ, p53, NOTCH1 and BRAF gene in thyroid cancer and their clinical relevance

QI Long 1 , ZUO Di 2, WANG Hui 1 , LI Xiao-tao 2   

  1. 1. Department of Nuclear Medicine, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200092, China; 2. Institute of Biomedical Sciences,East China Normal University, Shanghai 200241, China
  • Received:2012-01-01 Revised:2012-04-01 Online:2012-11-25 Published:2013-01-11

摘要: 旨在探讨〖WTBX〗REGγ,p〖STBX〗53,NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗在乳头状甲状腺癌中的表达及其与临床病理因素的相关性.应用Realtime-PCR及免疫组织化学方法检测〖WTBX〗REGγ,p〖STBX〗53,NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗在乳头状甲状腺癌组织中的表达,分析〖WTBX〗REGγ〖WTBZ〗与〖WTBX〗〖STBX〗p53,NOTCH1〖STBZ〗,BRAF〖WTBZ〗之间的关系及其与临床病理因素的相关性.〖WTBX〗REGγ〖WTBZ〗在乳头状甲状腺癌组织中表达明显增高,且其阳性率为87.5%(35/40).〖WTBX〗REGγ〖WTBZ〗表达与患者年龄、淋巴结转移情况有关(〖WTBX〗P〖WTBZ〗=0.015 1<0.05),与患者性别无显著性相关(〖WTBX〗P〖WTBZ〗=0.742>0.05);〖WTBX〗REGγ〖WTBZ〗表达与〖WTBX〗〖STBX〗p53,NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗表达呈正相关(〖WTBX〗P〖WTBZ〗=0.023 3<0.05).〖WTBX〗REGγ〖WTBZ〗阳性表达可作为乳头状甲状腺癌发生、发展的生物学指标,联合〖WTBX〗〖STBX〗p53〖WTBZ〗、〖WTBX〗〖STBX〗NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗检测可以为临床诊治及预后判断提供参考.

关键词: 甲状腺癌, p53, NOTCH1, REG γ, BRAF

Abstract: This study was designed to investigate the expressions of〖WTBX〗 REGγ〖WTBZ〗,〖WTBX〗 〖STBX〗p53〖WTBZ〗, 〖WTBX〗NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in papillary thyroid cancer as well as the potential correlations with clinicopathological significance. The expressions of 〖WTBX〗REGγ〖WTBZ〗,〖WTBX〗 p53〖WTBZ〗,〖WTBX〗 NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in thyroid cancer samples were detected by Realtime-PCR and immunohistochemistry, respectively. The correlations with clinicopathological factors were statistically analyzed. The expression of 〖WTBX〗REGγ〖WTBZ〗 in papillary thyroid cancer samples was significantly higher, with an 87.5% (35/40) positive rate in cancer tissues, and was correlated with age and lymph node status (〖WTBX〗P〖WTBZ〗=0.015 1<0.05), but not related to sex (〖WTBX〗P〖WTBZ〗=0.742>0.05). In addition, the 〖WTBX〗REGγ〖WTBZ〗 had a positive correlation with the levels of 〖WTBX〗〖STBX〗p53, NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 (〖WTBX〗P〖WTBZ〗=0.023 3<0.05), indicating that 〖WTBX〗REGγ〖WTBZ〗 could serve as an objective biological-marker for estimating the occurrence and progression of papillary thyroid cancer. Combined detection of 〖WTBX〗〖STBX〗p53〖WTBZ〗,〖WTBX〗 NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF 〖WTBZ〗may facilitate clinical diagnosis, treatment and prediction of prognosis.

Key words: thyroid cancer, p53, NOTCH1, REGγ, BRAF

中图分类号: