REGγ，p53，NOTCH1和BRAF在甲状腺癌中的表达及其临床意义

华东师范大学学报(自然科学版) ›› 20120, Vol. 2012 ›› Issue (6): 89-95.doi:

REG_γ，p53，NOTCH1和BRAF在甲状腺癌中的表达及其临床意义

亓龙¹，左娣²，王辉¹，李晓涛²

1.上海交通大学医学院附属新华医院核医学科，上海 200092； 2.华东师范大学生命医学研究所，上海 200241

收稿日期:2012-01-01 修回日期:2012-04-01 出版日期:2012-11-25 发布日期:2013-01-11

Expressions of REG_γ, p53, NOTCH1 and BRAF gene in thyroid cancer and their clinical relevance

QI Long ¹ , ZUO Di ²， WANG Hui ¹ , LI Xiao-tao ²

1. Department of Nuclear Medicine， Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine，Shanghai 200092, China； 2. Institute of Biomedical Sciences，East China Normal University， Shanghai 200241, China

Received:2012-01-01 Revised:2012-04-01 Online:2012-11-25 Published:2013-01-11

摘要/Abstract

摘要： 旨在探讨〖WTBX〗REGγ，p〖STBX〗53，NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗在乳头状甲状腺癌中的表达及其与临床病理因素的相关性.应用Realtime-PCR及免疫组织化学方法检测〖WTBX〗REGγ，p〖STBX〗53，NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗在乳头状甲状腺癌组织中的表达，分析〖WTBX〗REGγ〖WTBZ〗与〖WTBX〗〖STBX〗p53，NOTCH1〖STBZ〗，BRAF〖WTBZ〗之间的关系及其与临床病理因素的相关性.〖WTBX〗REGγ〖WTBZ〗在乳头状甲状腺癌组织中表达明显增高，且其阳性率为87.5%(35/40).〖WTBX〗REGγ〖WTBZ〗表达与患者年龄、淋巴结转移情况有关（〖WTBX〗P〖WTBZ〗=0.015 1<0.05），与患者性别无显著性相关（〖WTBX〗P〖WTBZ〗=0.742>0.05）；〖WTBX〗REGγ〖WTBZ〗表达与〖WTBX〗〖STBX〗p53，NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗表达呈正相关（〖WTBX〗P〖WTBZ〗=0.023 3<0.05）.〖WTBX〗REGγ〖WTBZ〗阳性表达可作为乳头状甲状腺癌发生、发展的生物学指标，联合〖WTBX〗〖STBX〗p53〖WTBZ〗、〖WTBX〗〖STBX〗NOTCH1〖STBZ〗〖WTBZ〗和〖WTBX〗BRAF〖WTBZ〗检测可以为临床诊治及预后判断提供参考.

关键词: 甲状腺癌, p53, NOTCH1, REG _γ, BRAF

Abstract: This study was designed to investigate the expressions of〖WTBX〗 REGγ〖WTBZ〗,〖WTBX〗〖STBX〗p53〖WTBZ〗, 〖WTBX〗NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in papillary thyroid cancer as well as the potential correlations with clinicopathological significance. The expressions of 〖WTBX〗REGγ〖WTBZ〗,〖WTBX〗 p53〖WTBZ〗,〖WTBX〗 NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in thyroid cancer samples were detected by Realtime-PCR and immunohistochemistry, respectively. The correlations with clinicopathological factors were statistically analyzed. The expression of 〖WTBX〗REGγ〖WTBZ〗 in papillary thyroid cancer samples was significantly higher, with an 87.5% (35/40) positive rate in cancer tissues, and was correlated with age and lymph node status (〖WTBX〗P〖WTBZ〗=0.015 1<0.05), but not related to sex (〖WTBX〗P〖WTBZ〗=0.742>0.05). In addition, the 〖WTBX〗REGγ〖WTBZ〗 had a positive correlation with the levels of 〖WTBX〗〖STBX〗p53, NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 (〖WTBX〗P〖WTBZ〗=0.023 3<0.05), indicating that 〖WTBX〗REGγ〖WTBZ〗 could serve as an objective biological-marker for estimating the occurrence and progression of papillary thyroid cancer. Combined detection of 〖WTBX〗〖STBX〗p53〖WTBZ〗,〖WTBX〗 NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF 〖WTBZ〗may facilitate clinical diagnosis, treatment and prediction of prognosis.

Key words: thyroid cancer, p53, NOTCH1, REG_γ, BRAF

中图分类号:

R736.1

亓龙，左娣，王辉，李晓涛. REG_γ，p53，NOTCH1和BRAF在甲状腺癌中的表达及其临床意义[J]. 华东师范大学学报(自然科学版), 20120, 2012(6): 89-95.

QI Long, ZUO Di， WANG Hui, LI Xiao-tao . Expressions of REG_γ, p53, NOTCH1 and BRAF gene in thyroid cancer and their clinical relevance[J]. Journal of East China Normal University(Natural Sc, 20120, 2012(6): 89-95.

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