以抗旱性较强的甘蓝型油菜Holiday为材料，在开花初期对油菜进行干旱胁迫处理，采用RTqPCR技术分析ABA2、BnSOS2、BnCS、CAM、CBF4、PIP1这6个油菜抗旱相关基因在干旱胁迫第1天、3天、5天、7天在根、茎、叶、花和青荚中的表达量.结果表明，干旱胁迫下，6个抗旱相关基因在油菜的不同器官中均出现了上调表达；在不同干旱胁迫下，各基因表达量呈现不同的变化趋势；在相同的器官中，各基因的表达量存在明显的不同，累积表达量表现为根中ABA2最大、CBF4最小，茎中CAM最大、CBF4最小，叶中PIP1最大、ABA2最小，花中CBF4最大、BnSOS2最小，青荚中BnCS最大，CBF4最小.说明植物在受到干旱胁迫时，不同的抗旱途径对干旱胁迫的响应程度是不同的；不同器官中各抗旱相关基因与胁迫时间的相关性分析表明，CAM基因在茎中的表达量、CBF4基因在花中的表达量与胁迫时间呈显著正相关.
The high droughttolerant variety Holiday was treated as the experimental material under drought stress in the early flower stage. The expression level of 6 droughtrelated genes (ABA2、BnSOS2、BnCS、 CAM、CBF4、PIP1) which were selected from different organs (root, stem, leaf, flower and green pod) at different stress times (1,3,5,7 days) were analyzed by qRT PCR technology. The result showed that the expression of six droughtresistant genes in different organs all increased under drought stress, and they presented different trends respectively under different drought stress. In the same organs, the expression of the six droughtresistant genes had obvious different. In root, the maximum cumulative expression value gene was ABA2, and the minimum cumulative expression value gene was CBF4; in stem, the maximum was CAM, the minimum was CBF4; in leaf, the maximum was PIP1, the minimum was ABA2; in flower, the maximum was CBF4, the minimum was BnSOS2; in green pod, the maximum was BnCS, the minimum was CBF4. It suggested that different drought resistance pathway played〖JP2〗 different role under drought stress. The correlation analysis between expression values of droughtrelated genes and stress time showed that the expression quantity of CAM gene in the stem volume, CBF4 gene in the flowers was significantly positively related to the stress time.
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中国综合性科技类核心期刊(北大核心)