华东师范大学学报(自然科学版) ›› 2014, Vol. 2014 ›› Issue (6): 109-120.doi: 10.3969/j.issn.1000-5641.2014.06.015

• 生命科学 • 上一篇    下一篇

原花青素在酸性条件下交联制备心脏瓣膜的性能研究

 王晓亚1,2, 马旭兵1,2, 翟万银2, 张嘉敏3, 朱自严3, 张红锋1, 常江2   

  1. 1. 华东师范大学 生命科学学院, 上海 200062 2. 中国科学院 上海硅酸盐研究所 生物材料与组织工程中心,上海 200050 3. 上海市血液中心 上海市输血研究所, 上海 200051
  • 出版日期:2014-11-25 发布日期:2015-02-07
  • 通讯作者: 张红锋, 女, 副教授, 研究方向为细胞生物学. 翟万银, 男, 副研究员,研究方向为生物材料与组织工程. E-mail:hfzhang@bio.ecnu.edu.cn;zhaiwy@maid.sic.ac.cn.
  • 作者简介:第一作者:王晓亚, 女, 硕士研究生,研究方向为细胞生物学.
  • 基金资助:

    国家自然科学基金(31070870)

Crosslinking of decellularized heart valve by procyanidins in the acidic buffer solution: Compliance, anti-calcification and biological compatibility

 WANG  Xiao-Ya1,2, MA  Xu-Bing1,2, DI  Wan-Yin2, ZHANG  Jia-Min3, ZHU  Zi-Yan3, ZHANG  Hong-Feng1, CHANG  Jiang2   

  1. 1. School of Life Science, East China Normal University, Shanghai 200062 China 2. Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics Chinese Academy of Sciences, Shanghai}\quad 200050, China 3. Shanghai Blood Center, Shanghai Institute of Blood Transfusion, Shanghai}\quad 200051, China  
  • Online:2014-11-25 Published:2015-02-07

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摘要： 为了明确原花青素procyanidins, PC)在酸性条件下交联脱细胞心脏瓣膜是否能够维持其柔顺度、抑制其钙化成核和具备良好的生物学特性, 以1 mg/mL PC~在酸性条件下(pH3.0~7.4)交联脱细胞心脏瓣膜,采用万能力学测试仪测试研究其柔顺度,并用体外钙化、体外酶降解、细胞黏附、抗凝血和血小板黏附实验研究其抗钙化和相关生物学特性.结果显示,弱酸性条件下交联的脱细胞瓣膜(PC1-pH6.0)呈现出近似于未交联脱细胞瓣膜的柔软程度,交联后拉伸强度显著提高(5.95±0.6) MPa vs (4.64±0.81)MPa, 同时弹性模量与未交联对照组相当, 显示出较好的柔顺度;PC1-pH6.0组体外可有效抑制瓣膜钙化;PC交联能保护脱细胞瓣膜不被II型胶原酶降解,
瓣膜间质细胞在交联脱细胞瓣膜表面黏附良好,交联后的脱细胞瓣膜具有更好的抗血栓潜能. 因此,pH6.0条件下PC交联的脱细胞心脏瓣膜具有类似于未交联脱细胞瓣膜的柔顺性,能有效抑制钙化, 具有抗酶降解、抗血栓和良好的细胞相容性,有望用作可再细胞化的生物瓣.

关键词: 原花青素, 交联, 柔顺度, 酸性条件, 抗钙化, 生物相容性

Abstract: The aim of this study was to explore whether procyanidins (PC)-crosslinked acellular heart valves in acidic buffer solution can obtain the identical matrix compliance similar to non-crosslinked acellular ones with anti-calcification and good biocompatibility properties. Based on the different pH acidic buffer
solutions of 1 mg/mL PC, the crosslinked acellular valves were crosslinked. The compliance was determined by bending angle measurement, anti-calcification by soaking in simulated body fluid, resistance to enzymatic degradation by collagenase-Ⅱ hydrolysis. Under pH 6.0 condition, PC crosslinked valve (PC1-pH 6.0) group was as soft as its non-crosslinked counterpart. Compared with non-crosslinked group (4.64 MPa), the ultimate tensile strength of
PC1-pH 6.0 group reached up to 5.95 MPa while the elastic modulus maintained the same, showing higher tensile strength than and the same compliance as the decellularized valves. Calcification was completely inhibited after crosslinking at pH 6.0. Under this condition, the PC-crosslinked acellular valves can effectively resistant to proteolysis compatible to glutaraldehyde-crosslinked ones. Furthermore, acellular valves in PC1-pH 6.0 group provided a good substance for adhesion of heart valve interstitial cells and present anti-thrombosis potential. PC-crosslinked acellular valves in the Acidic Buffer Solution (pH 6.0) possess identical matrix compliance with non-crosslinked decellularized valves, potential of anti-calcification, resistance to enzymatic degradation,
anti-thrombosis and good cytocompatibility, which may be used for preparing bioprosthetic heart valves.

Key words: procyanidins, crosslinking, compliance, acidic condition, anti-calcification, biological compatibility

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