华东师范大学学报(自然科学版) ›› 2006, Vol. 2006 ›› Issue (2): 7-12.

• 化学 • 上一篇    下一篇

毛细管电泳-安培法测定酵母菌中的RNA

陈 相, 王清江, 丁 飞, 吕 金, 何品刚, 方禹之   

  1. 华东师范大学 化学系,上海 200062
  • 收稿日期:2005-03-16 修回日期:2005-04-25 出版日期:2006-03-25 发布日期:2006-03-25
  • 通讯作者: 何品刚

Determination of Content of RNA in Yeast by Capillary Zone Electrophoresis with Amperometric Detection(Chinese)

CHEN Xiang, WANG Qing-jiang, DING Fei,LU Jin, HE Pin-gang, FANG Yu-zhi   

  1. Department of Chemistry, East China Normal University, Shanghai 200062, China
  • Received:2005-03-16 Revised:2005-04-25 Online:2006-03-25 Published:2006-03-25
  • Contact: HE Pin-gang

摘要： 报道了通过毛细管电泳-安培检测法测定腺嘌呤(A)和鸟嘌呤(G)来间接测定酵母菌中核糖核酸含量的方法.探讨了电极电位、运行缓冲溶液、分离电压及进样时间等对毛细管电泳分离-安培检测的影响,得到了较为优化的测定条件.在30 mmol/L 的硼酸盐缓冲液(pH 9.0)中,上述两组分可在18 min 内完全分离.腺嘌呤和鸟嘌呤的检测限分别为2.0×10-7mol/L和1.7×10-7 mol/L.该法具有较好的重现性和灵敏度，可以用于食品中酵母菌中核糖核酸的快速测定.

关键词: 毛细管电泳, 安培检测, 酵母菌, 核糖核酸, 毛细管电泳, 安培检测, 酵母菌, 核糖核酸

Abstract: The indirect determination of the content of RNA in yeast by analyzing adenine (A) and guanine (G) with capillary electrophoresis with amperometric detection (CE-AD) was reported. The effects of potential of working electrode, running buffer, separation voltage and injection time on the CE-AD determination were investigated. Adenine and guanine could be well separated in a 30 mmol/L borate buffer (pH 9. 0) within 18 min under the optimum conditions. Their detection limits of adenine (S/ N = 3) of 2.0 ×10-7 mol/ L and 1.7 ×10 -7mol/ L, respectively. The experimental results showed that this method is of high sensitivity and high repeatability, and potential to be used in the quick analysis of yeast in foods.

Key words: amperometric detection, yeast, ribonucleic acid (RNA), capillary electrophoresis, amperometric detection, yeast, ribonucleic acid (RNA)

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