华东师范大学学报(自然科学版) ›› 2006, Vol. 2006 ›› Issue (2): 98-104,.

• 生命科学 • 上一篇    下一篇

拟南芥VHA-c3基因的特异性表达和调节

徐 萍, 李小方, 曾卫军, 王水平   

  1. 华东师范大学 生命科学学院, 上海 200062
  • 收稿日期:2005-04-08 修回日期:2005-07-07 出版日期:2006-03-25 发布日期:2006-03-25
  • 通讯作者: 王水平

Regulation and Tissue Specific Expression of VHA-c3 of Arabidopsis Thaliana(Chinese)

XU Ping, LI Xiao-fang, ZENG Wei-jun, WANG Shui-ping   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2005-04-08 Revised:2005-07-07 Online:2006-03-25 Published:2006-03-25
  • Contact: WANG Shui-ping

摘要：

利用RT-PCR技术检测VHA-c基因在拟南芥中的表达，结果表明VHA-c3基因在拟南芥的果荚、花、叶、茎和根中都有表达，但是，在叶中的表达量远远高于其它的组织.以GUS基因作为报告基因构建了不同长度的VHA-c3基因启动子缺失突变体，利用农杆菌介导的瞬时表达系统检测GUS基因的表达，研究发现在VHA-c3基因起始密码子上游2812-2 234 bp之间的区域內存在着控制VHA-c3基因高表达的转录调控元件.

关键词: V-ATPase, c亚基, VHA-c3基因, 启动子, V-ATPase, c亚基, VHA-c3基因, 启动子

Abstract:

Gene expression of VHA-c was detected in Arabidopsis silique, flower, leaf, bolt, root. However, the expression level of VHA-c3 was much higher in leaves than in the other tissues. Analysis of the putative promoter of VHA-c3 was carried out in tobacoo leave system. A series of the promoter mutants were constructed in which various extents of VHA-c3 promoter were placed upstream of the GUS gene in vector pC1305.1. The expression of GUS was analyzed by Agrobacterium-mediated transient expression system.It was found that a 2 812 bp promoter region has high gene expression capacity. And there is a high potential key cis-element for leaf-specific expression between 2812-2 234 bp region.

Key words: c subunit, VHA-c3, promoter, V-ATPase, c subunit, VHA-c3, promoter

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