华东师范大学学报(自然科学版) ›› 2006, Vol. 2006 ›› Issue (4): 16-22.

• 物理学 电子科学技术 • 上一篇    下一篇

脉冲电场对中国红豆杉细胞的影响

黄琳玲1, 叶 红1, 陈树德1, 钟建江2   

  1. 1.华东师范大学 光谱学与波谱学教育部重点实验室，华东师范大学 物理系，上海 200062; 2.华东理工大学 生物反应器工程国家重点实验室，上海 200237
  • 收稿日期:2004-12-24 修回日期:2005-04-16 出版日期:2006-07-25 发布日期:2006-07-25
  • 通讯作者: 陈树德

Effect of Pulsed Electric Field on the Suspension Cultures of Taxus chinensis(Chinese)

HUANG Lin-ling1, YE Hong1, CHEN Shu-de1, ZHONG Jian-jiang2   

  1. 1.Key Laboratory of Optical and Magnetic Resonance Spectroscopy, Minsitry of Education, Department of Physics, East China Normal University,Shanghai 200062,China；  2.State Key Laboratory of Bioreactor Engineering, East ChinaUniversity of Science and Technology, Shanghai 200237, China
  • Received:2004-12-24 Revised:2005-04-16 Online:2006-07-25 Published:2006-07-25
  • Contact: CHEN Shu-de

摘要： 通过二维电泳（2-DE）技术来研究脉冲电场作用后48 h中国红豆杉细胞在蛋白表达上的变化.共进行了3次二维电泳重复实验，经过相关因子分析证实具有较高的重复性.比较对照组和经过脉冲电场处理的实验组的二维电泳图谱，发现有较明显的差异.用基质辅助激光解析-飞行时间质谱和液相色谱-电喷雾质谱对其中的部分蛋白进行分析鉴定.鉴定结果表明：核糖核酸蛋白粒、醛糖差向异构酶和铁氧还蛋白-NADP(+)还原酶这三个蛋白可能是与脉冲电场诱导相关的蛋白.

关键词: 脉冲电场, 中国红豆杉细胞, 二维电泳, 基质辅助激光解析-飞行时间质谱, 液相色谱-电喷雾质谱, 脉冲电场, 中国红豆杉细胞, 二维电泳, 基质辅助激光解析-飞行时间质谱, 液相色谱-电喷雾质谱

Abstract: Two dimensional gel electrophoresis(2-DE) was used to analyse changes of the protein expression of suspension cultures of Taxus chinensis 48 hours after pulsed electric field (PEF) elicitation. The 2-DE experiments were repeated for 3 times, and proved to be reliable according to the correlation coefficient analysis. Distinct changes were found from comparison of the protein patterns of control and PEF treated cells. Matrixassisted laser desorption/ionization mass spectrometry and electrospray ionization mass spectrometry were used to analyse some proteins. The results indicated that heterogeneous nuclear ribonucleoprotein(hnRNP), aldose l-epimerase family protein and Ferredoxin-NADP(+) reductase, putative/adrenodoxin reductase are related to the induction of PEF.

Key words: Taxus chinensis, two dimensional gel electrophoresis, matrix-assisted laser desorption/ionization mass spectrometry, electrospray ionization mass spectrometry, pulsed electromagnetic field (PEF), Taxus chinensis, two dimensional gel electrophoresis, matrix-assisted laser desorption/ionization mass spectrometry, electrospray ionization mass spectrometry

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