华东师范大学学报(自然科学版) ›› 2007, Vol. 2007 ›› Issue (4): 119-123.

• 生命科学 • 上一篇    下一篇

α-硫辛酸对小牛血管细胞氧化损伤的保护作用

廖德丰, 陈季武, 谢 萍, 朱振勤, 徐 容   

  1. 华东师范大学 生命科学学院,上海 200062
  • 收稿日期:2006-03-02 修回日期:2006-05-11 出版日期:2007-07-25 发布日期:2007-07-25
  • 通讯作者: 徐容

Effects of Lipoic Acid on Protecting in vitro CulturedBovine Aortic Cells from Oxidative Damage(Chinese)

LIAO De-feng, CHEN Ji-wu, XIE Ping, ZHU Zhen-qin, XU Rong   

  1. School of Life Science, East China Normal University, Shanghai 200062,China
  • Received:2006-03-02 Revised:2006-05-11 Online:2007-07-25 Published:2007-07-25

摘要： 为了研究α-硫辛酸（lipoic acid, LA）对心血管氧化损伤的保护作用，本研究以离体培养的小牛主动脉血管内皮细胞和平滑肌细胞为实验材料，探讨了LA 对这两种细胞氧化损伤的影响.结果表明，在H2O2胁迫下，LA可以剂量依赖性地提高细胞内的超氧化物歧化酶（SOD）活性，抑制细胞内过氧化产物丙二醛（MDA）的产生，提示LA对小牛主动脉血管内皮细胞和平滑肌细胞氧化损伤有保护作用.本研究为LA的保健和药用价值开发,特别是对LA在保护心血管免受氧化损伤的功效的研发，提供了自由基生物学实验依据.

关键词: α-硫辛酸, 内皮细胞, 平滑肌细胞, 超氧化物歧化酶, 丙二醛, α-硫辛酸, 内皮细胞, 平滑肌细胞, 超氧化物歧化酶, 丙二醛

Abstract: In order to reveal the potential function of lipoic acid （LA）in protecting cardiovascular system from oxidative damage，the effects of LA on cultured bovine aortic endothelial cells (EC) and smooth muscle cells (SMC) injured by H2O2 were investigated. The results showed that LA can dose-dependently promote the activities of superoxide dismutase (SOD) and decrease the level of malondialdehyde (MDA) in EC and SMC，indicating LA can effectively prevent EC and SMC from H2O2 induced oxidative damage. Our results can provide new free radical biological data for the further study on the medical and health-care use of LA.

Key words: endothelial cells, smooth muscle cells, SOD, MDA, lipoic acid, endothelial cells, smooth muscle cells, SOD, MDA

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