华东师范大学学报(自然科学版) ›› 2008, Vol. 2008 ›› Issue (6): 116-126.

• 生命科学 • 上一篇    下一篇

复合环境离子强度对BDCP/DNA的粒径与转染效率的影响

夏春霞1，刘健2,3，王依婷2，包艳洁1，王小霞2，王镜2，俞磊2   

  1. 1.华东师范大学 生命科学学院, 上海200062； 2.华东师范大学 生物医学工程与技术研究院, 上海200062; 3. Nitto Denko Technical Corp, CA 92058, USA
  • 收稿日期:2008-03-05 修回日期:2008-05-03 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 俞磊

Effect of ionic strength on the particle size and gene transfection efficiency of BDCP/DNA complexes(Chinese)

XIA Chun-xia1,LIU Jian2,3，WANG Yi-ting2,BAO Yan-jie1,WANG Xiao-xia2,WANG Jing2,YU Lei2   

  1. 1. School of Life Science, East China Normal University, Shanghai 200062,China; 2. Institute of Biomedical Engineering and Technology, East China Normal University, Shanghai 200062,China; 3. Nitto Denko Technical Corp, CA 92058, USA
  • Received:2008-03-05 Revised:2008-05-03 Online:2008-11-25 Published:2008-11-25

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摘要： 为了探讨组装环境对基因载体/DN复合物的粒径和转染效果的影响，在三种不同离子浓度溶液体系(PBS, 5% 葡萄糖溶液, H2O)中测量BDCP（biodegradable cationic polymer，一种生物可降解的阳离子聚合物）/DNA复合物粒径和结合力，进行了体外转染试验和毒性试验.结果显示，PBS最适合组装转染复合物，可取得更好的稳定性、最高的转染效率和较低细胞毒性、低溶血率；在5%葡萄糖溶液和水中组装的BDCP/质粒复合物结合力较弱，转染效率比较低.得出结论,BDCP/DNA粒径、结合力和基因转染效率受组装体系的离子强度影响.

关键词: 聚乙烯亚胺, 基因转染, 非病毒载体, 聚乙烯亚胺, 基因转染, 非病毒载体

Abstract: To explore the effects of particle formation environments on gene carrier-DNA particle size and gene delivery efficiency, the particle size, binding affinity were measured of BDCP (cationic polymer, having PEI 600 backbone with biodegradable bond) in varied ionic conditions(PBS, 5% glucose, H2O). And the gene delivery efficiency and cytotoxicity were tested in similar conditions. Results showed that PBS was favorable to achieve improved stability, high gene delivery efficiency and low hemolysis effect, and BDCP/plasmid combinded in 5% glucose or H2O were found weaker binding affinity and lower transfection efficiency. Cationic carrier/DNA particle size, affinity, and gene transfection efficiency are affected by ionic strength.

Key words: gene delivery, non-viral vector, polyethyleneimine, gene delivery, non-viral vector

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