华东师范大学学报(自然科学版) ›› 2009, Vol. 2009 ›› Issue (2): 72-77.

• 环境工程 地理学 • 上一篇    下一篇

白腐真菌产木质素降解酶及固定化载体研究

高尚1，陈诚1，陶芳1，戴兴春1，黄民生1，施华宏1，王国华2
  

  1. 1. 华东师范大学 资源与环境科学学院，上海200062； 2. 上海市政工程设计研究总院科研所，上海200092
  • 收稿日期:2008-02-03 修回日期:2008-05-09 出版日期:2009-03-25 发布日期:2009-03-25
  • 通讯作者: 黄民生

Ligninolytic enzymes production in white rot fungi and theusage of immobilized carriers （Chinese）

GAO Shang1,CHEN Cheng1,TAO Fang1,DAI Xing-chun1,HUANG Min-sheng1,SHI Hua-hong1,WANG Guo-hua2
  

  1. 1. School of Resource and Environment Science, East China Normal University, Shanghai 200062，China； 2. Shanghai Municipal Engineering Design Institute，Shanghai 200092，China
  • Received:2008-02-03 Revised:2008-05-09 Online:2009-03-25 Published:2009-03-25
  • Contact: HUANG Min-sheng

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摘要： 从四种白腐真菌中优选出最适产酶菌株黄孢原毛平革菌（〖WTBX〗Phanerochaete chrysosporium〖WTBZ〗），并对其在不同载体的附着及产酶情况进行了相关研究.试验结果表明，白腐真菌〖WTBX〗Phanerochaete chrysosporium〖WTBZ〗的锰过氧化物酶（MnP）及木质素过氧化物酶（LiP）活性最高分别可达225.67 U/L、110.35 U/L，较其它三种菌株要高；〖WTBX〗Phanerochaete chrysosporium〖WTBZ〗对尼龙纤维、厨用金刚砂尼龙、纤维海绵、玉米芯及海绵有较好的附着能力，但以厨用金刚砂尼龙、纤维海绵和玉米芯为最佳，而以玉米芯为载体所产生的MnP和LiP活性为最高，分别可达244.94 U/L和263.71 U/L，较未加载体的酶活有所提高且持续时间较长.实验优选出白腐真菌〖WTBX〗Phanerochaete chrysosporium〖WTBZ〗，且其以玉米芯为载体时所产MnP及LiP活性为最高.

关键词: 白腐真菌, 黄孢原毛平革菌, 固定化, 载体, 白腐真菌, 黄孢原毛平革菌, 固定化, 载体

Abstract: The adherence performance and enzymesproducing of four species of white rot fungi to different support materials were studied. The results indicated that the MnP and LiP of 〖WTBX〗Phanerochaete chrysosporium〖WTBZ〗 were higher than the other white rot fungi, with the maximum actirities up to 225.67 U/L, 110.35 U/L respectively. It could adhere well to the surfaces of nylon fibre, carborundum nylon, fibre sponge, corn cob and sponge. But the carriers of carborundum nylon, fibre sponge and corn cob were better than other carriers. The enzyme activity of the corn cob is higher and sustains longer. The peak activities of MnP and Lip were 244.94 U/L and 263.71 U/L, respectively.〖JP〗

Key words: Phanerochaete chrysosporium, immobilization, carrier , white rot fungi, Phanerochaete chrysosporium, immobilization, carrier

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