转化三七提取物为人参皂苷Rg3的真菌分离鉴定

华东师范大学学报(自然科学版) ›› 2011, Vol. 2011 ›› Issue (6): 115-121,151.

转化三七提取物为人参皂苷Rg₃的真菌分离鉴定

王青¹，袁萍²，茅仁刚²，王菊勇¹，郑展¹

1.上海中医药大学附属龙华医院中医肿瘤研究所，上海 200032；
2.上海师范大学生命与环境科学学院，上海 200234

收稿日期:2010-11-01 修回日期:2011-02-01 出版日期:2011-11-25 发布日期:2011-11-22

Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg₃

WANG Qing¹, YUAN Ping², MAO Ren-gang², WANG Ju-yong¹, ZHENG Zhan¹

1. Research Institute of Cancer, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200032, China;
2. School of Life Science, Shanghai Normal University, Shanghai 200234, China

Received:2010-11-01 Revised:2011-02-01 Online:2011-11-25 Published:2011-11-22

摘要/Abstract

摘要： 从培养人参(Panar ginseng C.A.Meyer)的土壤中筛选出23株菌,分别进行三七（Panax Notoginseng）提取物转化实验.经过摇瓶发酵后，采用TLC法与HPLC法对其发酵液提取物进行分析.研究结果表明，能够转化三七提取物为高活性人参皂苷的微生物菌株有9株，其中以菌株TR-20的转化作用最强.进一步对其发酵产物分析表明，菌株TR-20能够转化三七提取物为人参皂苷Rg₃，转化产物中人参皂苷Rg₃含量为190.2 mg/L，RSD为0.69%（n=5）.通过对菌株TR-20的形态和培养特征观察，表明菌株TR-20属于棒曲霉属（Aspergillus sp）.

关键词: 三七, 人参皂苷Rg₃, 菌种筛选, 菌种鉴定

Abstract: Twenty-three strains were isolated from soil where ginseng grew. The Panax Notoginseng extract were analyzed by TLC and HPLC. There were nine strains which can converte Panax Notoginseng extract into highly active ginsenoside, among which the TR-20 was the best one. Moreover, the TR-20 can convert Panax Notoginseng extract into ginsenoside Rg₃(190.2 mg/L). The TR-20 was Aspergillus (Aspergillus sp.) with observation of morphology and cultural characteristics.

Key words: Panax Notoginseng, ginsenoside Rg₃, strain screening, strain identification

中图分类号:

Q93-33

王青，袁萍，茅仁刚，王菊勇，郑展. 转化三七提取物为人参皂苷Rg₃的真菌分离鉴定[J]. 华东师范大学学报(自然科学版), 2011, 2011(6): 115-121,151.

WANG Qing, YUAN Ping, MAO Ren-gang, WANG Ju-yong, ZHENG Zhan. Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg₃[J]. Journal of East China Normal University(Natural Sc, 2011, 2011(6): 115-121,151.

参考文献

［1］ FENG Y L. Panax saponin and tumor ［J］. Foreign Med Sci Oncol, 2005, 32(9): 665-667.

［2］ LEE J Y, SHIN J W, CHUN K S. Antitumor promotional elfects of a novel intestinal bacterial metabolite (IH-901) derived from the protopanaxadiol-type ginsenosides in mouse skin ［J］. Carcinogenesis, 2005, 26(2): 359-367.

［3］赵文杰,陈迪,倪劲松,等. 20(S)-人参皂苷Rg3对前列腺癌PC-3M细胞的诱导凋亡作用［J］.中国药理学通报,2009,25(2):235-238.

ZHAO W J, CHEN D, NI J S, et al. Study on PC-3M cell apoptosis induced by 20(s)-ginsendoside Rg3 in prostate carcinoma［J］. Chinese Pharmacological Bulletin, 2009,25(2):235-238.

［4］〖JP2〗韩颖,姜彬慧,胡筱敏,等. Fusarium sacchari转化三七茎叶皂苷的稀有抗肿瘤成分［J］.中草药,2007,38(6):830-832.〖JP〗

HAN Y, JIANG B H, HU X M, et al. Fusarium sacchari transform rare anti-tumor composition in Panax Notoginseng stem and leaf saponin［J］. Chinese herbal medicine, 2007, 38(6): 830-832.

［5］ RUAN C C, ZHANG H, ZHANG L X. Biotransformation of ginsenoside Rf to Rh1 by recombinant β-glucosidase［J］. Molecules, 2009, 14: 2043-2048.

［6］ CHENG L Q, NA L R, BANG M H, et al. Conversion of major ginsenoside Rb1 to 20(S)-ginsenoside Rg3 by Microbacterium sp. GS514［J］. Phytochemistry, 2008, 69: 218-224.

［7］白龙律,臧蕴霞,尹成日.微生物转化人参皂苷Rb1为Rg3的研究［J］.延边大学学报:自然科学版,2009,35(2):141-144.

BAI L L, ZANG Y X, YIN C R. Microbiological transform ginsenoside Rb1 into Rg3［J］. Journal of Yanbian University: Natural Science Edition, 2009, 35(2): 141-144.

［8］ DONG A L, CUI Y J, GUO H Z. Microbiological transformation of ginsenoside Rg1［J］ . Journal of Chinese Pharmaceutical Sciences, 2001, 10(3): 115-118.

［9］付建国.人参皂苷微生物转化的研究［D］.长春:吉林农业大学,2004.

FU J G. The study of microbial transformation of ginsenoside［D］. Changchun: Jilin Agricultural University, 2004. ［10］宛晓春,汤坚,丁霄霖.β-葡萄糖苷酶产酶菌种的选育［J］.安徽农业大学学报,1997,24(1): 77-80.

WAN X C, TANG J, DING X L. Selection of an Aspergillus niger strain producing β-glucosidase［J］. Journal of Anhui Agricultural University, 1997, 24(1): 77-80.

［11］杨胜远,刘玉燕,梁志群.β-葡萄糖苷酶产生菌的分离筛选［J］.工业微生物,2002,32（4）,36-38.

YANG Y S, LIU Y Y, LIANG Z Q. Isolation and screening of β-glucosidase producing strain［J］. Industrial Microbiology, 2002, 32(4): 36-38.

［12］廖美德,许耀才,陈华癸.棒曲霉H-211的形态特征的研究［J］.湖北农学院学报,1995,15(2):102-104.

LIAO M D, XU Y C, CHEN H K. The morphological character of Aspergillus clavatus strain H-211［J］. Journal of Hubei Agricultural College, 1995, 15(2): 102-104.

［13］廖美德.棒曲霉H-211生理特性的研究［J］.湖北农学院学报,1997,17(4):279-282.

LIAO M D. Studies on the physiological properties of Aspergillus clavatus strain H-211［J］. Journal of Hubei Agricultural College, 1997, 17(4): 279-282.

转化三七提取物为人参皂苷Rg₃的真菌分离鉴定

Isolation and identification of the fungi which can convert Panax Notoginseng extract into ginsenoside Rg₃

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价