PFOS暴露对秀丽线虫寿长的影响及其与IIS相关基因的关联

doi:10.3969/j.issn.1000-5641.2015.02.013

华东师范大学学报(自然科学版) ›› 2015, Vol. 2015 ›› Issue (2): 106-112.doi: 10.3969/j.issn.1000-5641.2015.02.013

• “水体污染控制与治理”国家科技重大专项 • 上一篇下一篇

PFOS暴露对秀丽线虫寿长的影响及其与IIS相关基因的关联

李丹¹，武婧轩¹，李嘉^1,2，何德富^1,2

1. 华东师范大学生态与环境科学学院，上海200241；
2. 华东师范大学上海市城市化生态过程与生态恢复重点实验室，上海200241

收稿日期:2014-09-12 出版日期:2015-03-25 发布日期:2015-05-28
通讯作者: 何德富，男，博士，副教授，研究方向为毒理学、环境与健康等. E-mail:dfhe@des.ecnu.edu.cn
作者简介:李丹，女，硕士研究生，研究方向为环境污染与健康.
基金资助:
国家科技重大专项（2013ZX0731000104）；上海市自然科学基金（11ZR1410500）；河口海岸国家重点实验室开放课题（SKLECKF201311）

Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes

LI Dan ^1,2,WU Jing-xuan^1,2,LI Jia ^1,2,HE De-fu^1,2

1. School of Ecological and Environmental Sciences, East China Normal University, Shanghai200241, China;
2. Shanghai Key Lab for Urban Ecological Processes and Eco Restoration, East China Normal University, Shanghai200241, China

Received:2014-09-12 Online:2015-03-25 Published:2015-05-28

摘要/Abstract

摘要： 以秀丽隐杆线虫（C.elegans）为模型，本文研究了全氟辛烷磺酸（PFOS）对机体寿长的影响及初步机理.结果显示0.2~200 μmol/L的PFOS暴露50 h导致野生型秀丽线虫寿长呈剂量依赖性缩短.在4类转基因线虫上，观察到Insulin/IGF-l.1信号通路（IIS）相关的daf-16、daf-2和age-1基因突变或敲除能影响线虫的寿长.进一步观察PFOS暴露导致4类转基因线虫的寿长变化率，并与野生型线虫比较.在CF1139和CF1580突变种上daf16或daf2的突变均未改变PFOS的缩短寿长效应.而在CF1295和TJ1052转基因型上发现daf-16b的基因敲除或age-1基因突变阻断PFOS的减寿效应.结果表明PFOS慢性暴露能加速动物衰老，缩短寿长.PFOS作用与IIS信号通路关系密切，daf-16b和age-1基因在其中起重要作用.

关键词: PFOS, 秀丽隐杆线虫, 寿长, Insulin/IGF-l.1信号通路, daf-16b, age-1

Abstract: Using a model of C.elegans, this study aims to investigate the effect of PFOS on the lifespan and its mechanisms. Results showed that 0.2~200 μmol/L PFOS shortened the lifespan of wild type wormsin a concentrationdependent manner after 50 h exposure.In four transgenic strains, mutation of daf-16, daf-2 and age-1 which are related to Insulin/IGFl.1pathway(IIS), could affect the lifespans of nematodes. We further observed lifespan change rates of the four transgene C.elegansafter exposed to PFOS.Results showed that mutation of daf-16 or daf-2 did not alter PFOS induced effects of shortening lifespan in CF1139 and CF1580 strain.However, knockout of daf-16b or mutation of age-1blocked PFOS induced effects of shortening lifespan in CF1295 and TJ1052 strain. Our results suggested that PFOS could accelerate aging and shorten the lifespan of nematodes. PFOSinduced effects are closely relative with IIS signaling pathways, in which daf-16b and age-1 play important roles.

Key words: PFOS;C.elegans, lifespan, IIS signaling pathway, daf-16b, age-1

中图分类号:

X592

李丹, 武婧轩, 李嘉, 何德富. PFOS暴露对秀丽线虫寿长的影响及其与IIS相关基因的关联[J]. 华东师范大学学报(自然科学版), 2015, 2015(2): 106-112.

LI Dan, WU Jing-Xuan, LI Jia, HE De-Fu. Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes[J]. Journal of East China Normal University(Natural Sc, 2015, 2015(2): 106-112.

参考文献

［1］BUTENHOFF J L, OLSEN G W, PFAHLESHUTCHENS A. The applicability of biomonitoring data for perfluorooctanesulfonate to the environmental public health continuum［J］. Environ Health Perspect,2006, 114(11): 17761782.

［2］HOUDE M, MARTIN J W, LETCHER R J, et al. Biological monitoring of polyfluoroalkylsubstances: a review［J］. Environ Sci & Technol, 2006, 40(11): 34633473.

［3］刘薇，金一和，全燮，等. 沈阳市降雪中PFOS和PFOA污染现状调查［J］. 环境科学, 2007, 28(9):20682073.

［4］金一和，丁梅，翟成，等. 长江三峡库区江水和武汉地区地面水中PFOS和PFOA污染现状查［J］. 生态环境, 2006, 15(3): 486489.

［5］GIESY J P,KANNAN K. Peer reviewed: Perfluorochemical surfactants in the environment［J］. Environ Sci&Technol. 2002, 36(7): 146A152A.

［6］EHRESMAN D J,FROEHLICH J W,OLSEN G W, et al. Comparison of human whole blood, plasma,andserum matrices for the determination of perfluorooctanesulfonate (PFOS), perfluorooctanoate(PFOA), and other fluorochemicals［J］. Environ Res, 2007, 103(2): 176184.

［7］HOPF N B, RUDER A M, SUCCOP P. Background levels of polychlorinated biphenyls in the US population［J］. Sci Total Environ, 2009, 407(24): 61096119.

［8］OLSEN G W, BURRIS J M, EHRESMAN D J, et al. Halflife of serum elimination of perfluorooctanesulfonate, perfluorohexanesulfonate and perfluorooctanoate in retired fluorochemical production workers ［J］. Environ Health Perspect, 2007, 115(9): 1298.

［9］仇敬运，屈艾，胡文静，等. 全氟辛烷磺酸钾对小鼠肝脏的氧化性损伤研究［J］. 毒理学杂志, 2011（4）:265268.

［10］范轶欧，金一和，麻懿馨，等. 全氟辛烷磺酸对雄性大鼠生精功能的影响［J］. 卫生研究, 2005, 34(1): 3739

［11］JOHANSSONN, FREDRIKSSON A, ERIKSSON P. Neonatal exposure to perfluorooctanesulfonate (PFOS) and perfluorooctanoic acid (PFOA) causes neurobehavioural defects in adult mice ［J］.Neurotoxicology, 2008, 29(1): 160169.

［12］LIN K, HSIN H,LIBINA N, et al.Regulation of the Caenorhabditiselegans longevity protein DAF16 by insulin/IGF1 and germline signaling［J］. Nat Genet, 2001, 28(2):13945.

［13］LIBINAN, BERMAN J R, KENYON C. Tissuespecific activities of C. elegans DAF16 in the regulation of lifespan［J］. Cell, 2003,115: 489502.

［14］JOHNSON T E, TEDESCO P M, LITHGOW G J. Comparing mutants, selective breeding, and transgenics in the dissection of aging processes of Caenorhabditiselegans［J］. Genetica, 1993, 91: 6577.

［15］APFELD J, KENYON C. Cell nonautonomy of C.elegans daf2 function in the regulation of diapause and life span［J］. Cell,1998,95:199210.

［16］KANNAN K, YUN S H, RUDD R J, et al. High concentrations of persistent organic pollutants including PCBs, DDT, PBDEs and PFOS in little brown bats with whitenose syndrome in New York, USA［J］. Chemosphere, 2010, 80(6): 613618.

［17］PAIANO V, FATTORE E, CARRA A, et al. Liquid chromatographytandem mass spectrometry analysis of perfluorooctane sulfonate and perfluorooctanoic acid in fish fillet samples ［J］. J Anal Methods Chem, 2012, 2012: 719010.

［18］NAOKI O, RINNA N, TOMOKA M, et al. A secreted decoy of InR antagonizes insulin/IGF signaling to restrict body growth in Drosophila ［J］. Genes & Dev, 2013, 27: 8797.

［19］KENYON C J. The genetics of ageing ［J］. Nature, 2010, 464(7288): 504512.

［20］SCOTT F L, MARISSA F, ANISOARA B, et al. Lifespan extension from hypoxia in Caenorhabditis elegans requires both HIF1 and DAF16 and is antagonized by SKN1［J］. J GerontolA BiolSci Med Sci, 2013, 68: 11131144.

［21］ROBERT J S R, SRINIVAS A, W ALEX C, et al. Gene categories differentially expressed in C. elegans Age〖STBX〗1〖STBZ〗 mutants of extraordinary longevity: New insights from novel datamining procedures［J］. J Gerontol A BiolSci Med Sci, 2012, 67A: 366375.

[1]	杨永胜, 刘梦芸, 黄民生, 何德富. 杭州西湖龙泓涧和北里湖水体及底泥毒性的生物学评价[J]. 华东师范大学学报(自然科学版), 2015, 2015(2): 98-105.
[2]	刘俊奇;刘青坡;曹庆珍;钱丽娟;郭素珍;施华宏. 全氟辛磺酸对于爪蟾的甲状腺激素干扰效应[J]. 华东师范大学学报(自然科学版), 2010, 2010(4): 118-124.

PFOS暴露对秀丽线虫寿长的影响及其与IIS相关基因的关联

Effects of PFOS exposure on lifespan in C. elegans and their relations with IIS genes

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价