异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究

doi:10.3969/j.issn.1000-5641.2018.06.003

华东师范大学学报(自然科学版) ›› 2018, Vol. 2018 ›› Issue (6): 22-31,87.doi: 10.3969/j.issn.1000-5641.2018.06.003

异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究

张婷月^1,2, 丁钰^1,2, 黄民生^1,2

1. 华东师范大学生态与环境科学学院, 上海 200241;
2. 华东师范大学上海市城市化生态过程与生态恢复重点实验室, 上海 200241

收稿日期:2018-06-08 出版日期:2018-11-25 发布日期:2018-12-01
通讯作者: 黄民生,男,教授,博士生导师,研究方向为水环境治理与修复.E-mail:mshuang@des.ecnu.edu.cn. E-mail:mshuang@des.ecnu.edu.cn
作者简介:张婷月,女,硕士研究生,研究方向为水环境治理与修复.E-mail:1391994089@qq.com.
基金资助:
国家重大科技专项（2014ZX07101102，2017ZX07207001）；上海市普陀区高层次人才科研创新项目（2014-A-18）；地理信息科学教育部重点实验室开放研究基金（KLGIS2016A03）

Screening of heterotrophic nitrification-aerobic denitrifying bacteria and its nitrogen removal characteristics

ZHANG Ting-yue^1,2, DING Yu^1,2, HUANG Min-sheng^1,2

1. School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China;
2. Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, East China Normal University, Shanghai 200241, China

Received:2018-06-08 Online:2018-11-25 Published:2018-12-01

摘要/Abstract

摘要： 为寻求高效水体脱氮手段，从龙泓涧梯级塘底泥中筛选出以Pseudomonas菌属为主、具有异养硝化-好氧反硝化功能菌群，将其命名为LHJ-1.异养硝化和好氧反硝化性能研究结果表明，菌群LHJ-1具有明显的异养硝化功能，对NH₄⁺-N和TOC利用率分别达99.90%和56.69%，且表现出较高的反硝化能力，对NO₃^--N和NO₂^--N的转化率分别为92.46%和89.67%.由不同环境因素（碳氮比、碳源、pH值和溶解氧）影响实验可知，多种环境因子均对菌群LHJ-1脱氮效果具有较大影响，因此在实际应用中需考察不同环境因子，以找出最佳生长条件，获得最大脱氮效率.异养硝化-好氧反硝化菌群LHJ-1的筛选在水体脱氮除碳中具有广阔的应用前景.

关键词: 异养硝化, 好氧反硝化, 混合菌, 脱氮性能

Abstract: In order to find a high-efficiency denitrification method, a Pseudomonas genus-based strain was selected from the sediment of Cascade Pond in Longhong Ravine. The strain had heterotrophic nitrification and aerobic denitrification capability and was named LHJ-1. The results showed that the bacterial consortium LHJ-1 had clear heterotrophic nitrification capability; the utilization rates of NH₄⁺-N and TOC were 99.90% and 56.69%, respectively. Concurrently, it demonstrated good denitrification ability, with conversion rates of NO₃^--N and NO₂^--N at 92.46% and 89.67%, respectively. Experiments evaluating environmental effects showed that various factors (e.g., C/N ratio, carbon source, pH, and DO concentration) could have a significant influence on the denitrification effect. Accordingly, different environmental factors should be investigated in practical applications to determine the optimum growth conditions for the best nitrogen removal efficiency. The screening of heterotrophic nitrification and aerobic denitrifying bacteria consortium LHJ-1 has broad application prospects for both denitrification and decarbonization of water.

Key words: heterotrophic nitrification, aerobic denitrification, mixed bacterial community, denitrification characteristics

中图分类号:

X522

张婷月, 丁钰, 黄民生. 异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究[J]. 华东师范大学学报(自然科学版), 2018, 2018(6): 22-31,87.

ZHANG Ting-yue, DING Yu, HUANG Min-sheng. Screening of heterotrophic nitrification-aerobic denitrifying bacteria and its nitrogen removal characteristics[J]. Journal of East China Normal University(Natural Sc, 2018, 2018(6): 22-31,87.

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异养硝化-好氧反硝化细菌的筛选及其脱氮性能研究

Screening of heterotrophic nitrification-aerobic denitrifying bacteria and its nitrogen removal characteristics

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