关键词: 电磁场生物效应, 胰岛素的信号系统, 酪氨酸磷酸化, mRNA芯片检测, 电磁场生物效应, 胰岛素的信号系统, 酪氨酸磷酸化, mRNA芯片检测

Abstract: Insulin was exposed to the pulsed electric field (PEF) for 0 min or 20 min before being added to the culture medium containing L-02 human hepatocytes. Thirty minutes later, the tyrosine phosphorylation level of the cells for each group was monitored by a flow cytometry. The average fluorescent intensity of the test cell groups decreased by 10.33% compared with the control groups. After the cells were cultured in the medium with the exposed insulin or the normal insulin for 68 hours, the gene expression levels were measured by using a gene expression chip. The change of fifty-five out of 12,000 gene sequences of the test groups were obvious compared to the controls. The human tyrosine phophatase mRNA showed increases in their expressions. These results suggested that, the intercellular signal molecule such as insulin can be the target of the electromagnetic field, and acts as a signal carrier of the information of PEF, which consequently causes its biological effects.

Key words: electromagnetic field, signal pathway, tyrosine phosphorylation, gene expression, insulin, electromagnetic field, signal pathway, tyrosine phosphorylation, gene expression