华东师范大学学报(自然科学版) ›› 2005, Vol. 2005 ›› Issue (5/6): 183-187.

• 生命科学 • 上一篇    下一篇

氮源水解液对链霉菌转谷氨酰胺酶合成的影响

常忠义1，杨雪霞2，庄晓辉1，黄秀琴1，高红亮1   

  1. 1.华东师范大学 生命科学学院,上海200062;2.东华大学 化学与化工学院，上海200054
  • 收稿日期:2004-05-12 修回日期:2004-09-15 出版日期:2005-12-31 发布日期:2005-12-31
  • 通讯作者: 高红亮

Effects of the Hydrolysate of Nitrogen Sources on Transglutaminase by Streptomyces(Chinese)

CHANG Zhong-yi 1 ,YANG Xue-xia,ZHUANG Xiao-hui,HUANG Xiu-qin,GAO Hong-liang   

  1. 1. Schools of life science, East China Normal University, Shanghai200062, China;2. Colloge of Chemistry and Chemical Engieering,Donghua University,Shanghai200054,China
  • Received:2004-05-12 Revised:2004-09-15 Online:2005-12-31 Published:2005-12-31
  • Contact: GAO Hong-liang

摘要： 研究了常压及高压条件下以豆饼粉、棉籽粉酸水解液作氮源，对链霉菌（Streptomyces）HS-1产转谷氨酰胺酶的影响.初步试验高压条件下以所得豆饼粉水解物作氮源，链霉菌（streptomyces）HS-1产转谷氨酰胺酶的酶活可达3.86 μmol/(min•mL).对高压条件下豆饼粉的水解条件进行确定：以3 mol/L的盐酸水解4 h所得水解物的发酵酶活较高，为5.17 μmol/(min•mL)，且进一步实验以蛋白胨与豆饼粉水解液作混合氮源时发酵酶活较单氮源高.

关键词: 豆饼粉, 酸水解, 转谷氨酰胺酶, 豆饼粉, 酸水解, 转谷氨酰胺酶

Abstract: The effect of hydrochloric acid hydrolysis of soybean cake powder and cottonseed powder under different pressure were investigated on MTGase production by Streptomyces.The results showed that hydrolysate of soybean cake powder in high pressure served as favorable source.MTGase activity could reach 3.86 μmol/(min•mL).The optimum of hydrolysis of soybean cake powder is followed:suitable concentration of hydrochloric acid is 3 mol/L,hydrodysis time is 4 hours. The experimental results showed that the most suitable fermentation medium consisted of 1.0% peptone,1.5% hydrolysate of soybean cake powder.

Key words: acid hydrolysis, MTGase, soybean cake powder, acid hydrolysis, MTGase

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