华东师范大学学报(自然科学版) ›› 2004, Vol. 2004 ›› Issue (2): 92-96.

• 生命科学 环境科学 • 上一篇    下一篇

原花青素对•OH致损的鼠肝线粒体的保护作用

陈季武1, 朱振勤1, 苏 裕1, 王 妮1, 曹家琪1, 刘国伟1, 胡 斌1, 翟万垠2   

  1. 1.华东师范大学 生命科学学院，上海 200062; 2.中国科学院硅酸盐研究所 生物材料与组织工程中心，上海 200050; 2.Center for Bio-materials and Tissue Engineering,Shanghai Institutes of Ceramics,Chinese Academy of Science ,Shanghai 200050 China
  • 收稿日期:2003-02-21 修回日期:2003-06-11 出版日期:2004-06-25 发布日期:2004-06-25
  • 通讯作者: 陈季武

Effects of Procyanidins on Preventing Mitochondria Damage in Rat Liver Induced by •OH

CHEN Ji-wu1,ZHU Zhen-qin1,SU Yu1,WANG Ni1,CAO Jia-qi1,LIU Guo-wei1,HU Bing1, ZHAI Wan-yin2   

  1. School of Life Science,East China Normal University,Shanghai 200062 China
  • Received:2003-02-21 Revised:2003-06-11 Online:2004-06-25 Published:2004-06-25
  • Contact: CHEN Ji-wu

摘要： 采用分光光度法和荧光偏振法，检测了原花青素对•OH引起的鼠肝线粒体损伤的保护作用.结果表明，原花青素能有效增加受损伤的线粒体的膜脂流动性，明显减少受损伤的线粒体肿胀及脂质过氧化物的形成,从而维护线粒体结构和功能的完整性.以上结果为原花青素的进一步开发提供了新的资料.

关键词: 原花青素, 羟自由基, 线粒体, 线粒体肿胀, 脂质过氧化, 膜脂流动性, 原花青素, 羟自由基, 线粒体, 线粒体肿胀, 脂质过氧化, 膜脂流动性

Abstract: Oxidative damage of the mitochondria plays an important role in generating and developing process of many diseases.Hydroxyl radical (•OH) has been considered an important factor inducing mitochondria damage.Therefore,it has important significance to study and exploit medicines protecting mitochondria damage caused by •OH.We measured effects of procyanidins preventing mitochondria damage induced by•OH using spectrophotometry and fluorescent polarimetry.The results showed that procyanidins increased effectively membrane lipids fluidity of damaged mitochondria,decreased significantly the mitochondria swelling and lipid peroxidation product formation.As a result,procyanidins can maintain the integrity of structure and function of mitochondria.Above results can provide new data for further exploitation of procyanidins.

Key words: hydroxyl radical, mitochondria, mitochondria swelling, lipid peroxidation, membrane lipid fluidity, procyanidins, hydroxyl radical, mitochondria, mitochondria swelling, lipid peroxidation, membrane lipid fluidity

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