华东师范大学学报(自然科学版) ›› 2009, Vol. 2009 ›› Issue (6): 134-140.

• 生命科学 • 上一篇    

秀丽小杆线虫Caenorhabditis elegans pkc-2基因cDNA的克隆和表达

钱雨1,2,Laurent SGALAT2   

  1. 1. 华东师范大学 生命科学学院, 上海200062; 2. 克洛德•贝纳尔-里昂第一大学 细胞与分子遗传学中心, 法国 里昂69100
  • 收稿日期:2008-10-10 修回日期:2008-11-27 出版日期:2009-11-25 发布日期:2009-11-25
  • 通讯作者: 钱雨

Cloning and expression pattern analysis of a cDNA of pkc-2gene in Caenorhabditis elegans (Chinese, Notes)

QIAN Yu1，2,Laurent SGALAT2   

  1. 1. School of Life Science， East China Normal University, Shanghai200062, China; 2. Center for Cell and Molecular Genetics, Université Claude Bernard Lyon 1, Lyon 69100, France
  • Received:2008-10-10 Revised:2008-11-27 Online:2009-11-25 Published:2009-11-25
  • Contact: QIAN Yu

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摘要： 蛋白激酶C在秀丽小杆线虫中具有调节肌细胞渐进性萎缩的功能.为了揭示它的调节机制, 本研究克隆了秀丽小杆线虫中蛋白激酶C pkc-2基因的cDNA pkc-2-c，构建了含该pkc-2基因cDNA亚型的重组质粒pPD 118.20-pKG 63；揭示了该cDNA在秀丽小杆线虫体壁肌细胞中的定位.

关键词: 秀丽小杆线虫, pkc-2基因, cDNA克隆, 免疫细胞化学, 秀丽小杆线虫, pkc-2基因, cDNA克隆, 免疫细胞化学

Abstract: Protein kinase C regulates the progressive muscle degeneration in Caenorhabditis elegans. In order to investigate the function of PKC involved in muscle degeneration, this paper cloned a cDNA isoform of pkc-2 gene of C.elegans and constructed the recombinant plasmids pPD118.20-pKG63 containing the isoform. The new isoform was then further studied for gene expression pattern. Immunocytochemistry experiment showed that this cDNA isoform expressed in body-wall muscle cells and located near the dense body.

Key words: pkc-2 gene, clone, immunocytochemistry, Caenorhabditis elegans, pkc-2 gene, clone, immunocytochemistry