华东师范大学学报(自然科学版) ›› 2007, Vol. 2007 ›› Issue (2): 58-64.

• 生命科学 • 上一篇    下一篇

包囊游仆虫休眠细胞中大核和线粒体DNA的多态性

陈季武1， 曾 红1,2， 顾福康1   

  1. 1. 华东师范大学 生命科学学院， 上海 200062； 2. 福建师范大学 生物工程学院， 福州 350007
  • 收稿日期:2005-09-02 修回日期:2005-10-12 出版日期:2007-03-25 发布日期:2007-03-25
  • 通讯作者: 顾福康

Preliminary Study on Polymorphism of Macronuclear and Mitochondria DNA in Resting Cell of Euplotes encysticus(Chinese)

CHEN Ji-wu1, ZENG Hong1,2, GU Fu-kang1

  

  1. 1. School of Life Science, East China Normal University, Shanghai 200062, China; 2. School of Biology Engineering, Fu Jian Normal University, Fuzhou 310007, China
  • Received:2005-09-02 Revised:2005-10-12 Online:2007-03-25 Published:2007-03-25
  • Contact: GU Fu-kang

摘要： 为了探索纤毛虫休眠细胞中两套遗传系统的作用特征，对包囊游仆虫（Euplotes encysticus）休眠细胞与营养细胞大核DNA和线粒体DNA进行了RAPD比较.结果显示，在所选用的35条随机引物中，包囊游仆虫大核DNA共扩增出220条片段，其中以休眠细胞大核DNA为模板扩增出18条特有片段，以营养细胞大核DNA为模板扩增出44条特有片段，两者存在28%的差异.在所选用的32条随机引物中，线粒体DNA共扩增出154条片段，其中以休眠细胞线粒体DNA为模板扩增出19条特有片段，以营养细胞线粒体DNA为模板扩增出25条特有片段，两者有29%的差异.这些结果表明，包囊游仆虫休眠细胞与营养细胞的大核DNA结构存着一定的差异；两者的线粒体DNA结构也存在差异.因此，包囊游仆虫在休眠细胞形成过程中，大核DNA、线粒体DNA结构可能都发生了一定的变化，并且这些变化可能与休眠细胞形成过程中的形态结构和代谢活动等剧烈变化以及休眠状态下的生理生化变化密切相关.所得结果为揭示纤毛虫细胞结构的分化与细胞遗传物质的作用关系提供了基础资料.

关键词: 包囊游仆虫, 休眠细胞, 营养细胞, 大核DNA, 线粒体DNA, 包囊游仆虫, 休眠细胞, 营养细胞, 大核DNA, 线粒体DNA

Abstract: The macronuclear and mitochondria DNA between the resting and the vegetative cell of Euplotes encysticus were comparatively analyzed by using RAPD technique in order to explore the acting feature of two genetic systems in the resting cell of ciliates. The results showed that 220 fragments were amplified in the resting and the vegetative cell DNA from Euplotes encysticus (EE) by using 35 random primers. In these fragments, both the resting and the vegetative cell macronucleus DNA had 18 and 44 specific fragments, respectively. The variance values was 28%. And 154 fragments were amplified in the resting and the vegetative cell mitochondria DNA from EE by using 32 random primers. Among them, both the resting and the vegetative cell mitochondria DNA had 19 and 25 specific fragments, respectively. The variance values was 29%. These data hinted that the structures of the macronuclear DNA in the resting and the vegetative cell of EE had some variances. The structure of both mitochondria DNA also had some variances. Therefore, in the process of EE forming resting cell, the structures of the macronuclear and mitochondria DNA all had perhaps some changes. These changes were closely related to the acute alteration for structural features and metabolism activities during the resting cell formation and related to physiological and biochemical alteration in the resting state. These results provided basic data for revealing relationship between differentiation of ciliates cell structure and acting of cell genetic substance.

Key words: resting cell, vegetative cell, macronuclear DNA, mitochondria DNA, Euplotes encysticus, resting cell, vegetative cell, macronuclear DNA, mitochondria DNA

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