华东师范大学学报(自然科学版) ›› 2007, Vol. 2007 ›› Issue (2): 87-92.

• 生命科学 • 上一篇    下一篇

α-硫辛酸和二氢硫辛酸的抗氧化作用

廖德丰， 陈季武, 谢萍， 朱振勤, 徐容   

  1. 华东师范大学 生命科学学院，上海 200062
  • 收稿日期:2005-11-10 修回日期:2005-12-30 出版日期:2007-03-25 发布日期:2007-03-25
  • 通讯作者: 徐容

Studies on the Antioxidation Effects of Alpha-Lipoic Acid and Dihydrolipoic Acid(Chinese)

LIAO De-feng， CHEN Ji-wu， XIE Ping， ZHU Zhen-qin， XU Rong   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2005-11-10 Revised:2005-12-30 Online:2007-03-25 Published:2007-03-25
  • Contact: XU Rong

摘要：

采用多种化学发光体系和比色体系研究了氧化型硫辛酸——α-硫辛酸（α-lipoic acid, LA）和还原型硫辛酸——二氢硫辛酸（dihydrolipoic acid, DHLA）直接清除活性氧、抗脂质过氧化以及对•OH引起的DNA氧化损伤的保护作用.实验发现，LA和DHLA能有效清除相应体系中的活性氧过氧化氢（H2O2）、羟自由基（•OH）、过氧亚硝基阴离子（ONOO-）和二苯代苦味肼基自由基（DPPH• ），能显著抑制脂质过氧化，并对•OH引起的DNA氧化损伤有良好的保护作用，而DHLA还可以有效清除超氧阴离子自由基（O•2-）.这些结果提示，LA和DHLA都是良好的抗氧化剂，但相比之下DHLA的抗氧化能力要显著强于LA.本研究为LA和DHLA保健和药用价值的进一步开发，提供了自由基生物学方面的依据.

关键词: α-硫辛酸, 二氢硫辛酸, 抗氧化剂, 活性氧, DNA氧化损伤, α-硫辛酸, 二氢硫辛酸, 抗氧化剂, 活性氧, DNA氧化损伤

Abstract: Lipoic acid(LA) widely exists in all kinds of cells. It can be converted to dihydrolipoic acid (DHLA) in mammalian cell. The ability of alpha-LA and DHLA to scavenge reactive oxygen species and prevent DNA from oxidative damage by •OH was evaluated using chemiluminescence systems and colorimetric systems. The experiments show that LA and DHLA can directly scavenge •OH、H2O2、ONOO-and DPPH• ，effectively inhibit lipid peroxidation and DNA oxidative damage by •OH； DHLA can also effectively scavenge O•2-. These results indicate that both LA and DHLA are effective antioxidants and the anti-oxidative ability of DHLA is obviously stronger than that of LA. The present investigation can provide new free radical biological data for the further study on the medical and health-care applications of LA and DHLA.

Key words: dihydrolipoic acid, antioxidant, active oxygen species, DNA oxidative damage, chemiluminescence, lipoic acid, dihydrolipoic acid, antioxidant, active oxygen species, DNA oxidative damage, chemiluminescence

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