华东师范大学学报(自然科学版) ›› 2004, Vol. 2004 ›› Issue (1): 73-78.

• 生命科学 • 上一篇    下一篇

中华眼镜蛇心脏毒素对疼痛及记忆的影响

刘华清1, 李荣秀2, 肖齐士2, 刘浩然2, 翁恩琪1, 王霆3   

  1. 1.华东师范大学环境科学与技术系，上海 200062; 2.中科院上海生物工程研究中心，上海 200233; 3.威尔曼国际新药开发中心，广州 510075
  • 收稿日期:2002-12-15 修回日期:2003-03-02 出版日期:2004-03-25 发布日期:2004-03-25
  • 通讯作者: 刘华清

Effects of Chinese Cobra Cardiotoxin on Pain and Memory

LIU Hua-qing1, LI Rong-xiu2, XIAO Qi-shi2, LIU Hao-ran2, WENG En-qi1, WANG Ting3   

  1. 1. Department of Environmental Science and Technology, East China Normal University, Shanghai 200062, China 2. Shanghai Research Center of Biotechnology, Chinese Academy of Science，Shanghai 200233, China; 3. Shanghai Biomimetic Life Science Ltd., Shanghai 200233, China
  • Received:2002-12-15 Revised:2003-03-02 Online:2004-03-25 Published:2004-03-25
  • Contact: LIU Hua-qing

摘要：

通过热板法及醋酸扭体法观察中华眼镜蛇心脏毒素(CTX)对成年昆明种小鼠疼痛的影响，通过Y一型电迷宫及穿梭箱法研究CTX对东蓖若碱诱发成年昆明种小鼠学习记忆障碍的影响，利用跳台法研究CTX对东蓑若碱诱发成年BABL/C小鼠学习记忆障碍的影响，利用Morris水迷宫法研究CTX对对东蓖若碱诱发成年C57BL/6J小鼠学习记忆障碍的影响。实验发现，CTX能明显增加热刺激引起的疼痛闭值，明显减少醋酸引起的扭体次数，并能显著增加小鼠在Y型迷宫实验中的正确反应次数，减少在穿梭箱实验中小鼠重新训练至学会标准的训练次数及小鼠的反应时间，能增加小鼠在跳台法中的潜伏期，减少错误反应次数，减少小鼠在Morris水迷宫实验中的寻台时间和游泳路程，以上效果均表现出明显的剂量依赖性。以上结果提示，CTX有良好的镇痛作用以及对东莫若碱诱发的小鼠学习记忆障碍的改善作用，这可能是由于CTX与眼镜蛇神经毒素(NT)三维结构相似而导致了相似的生物学效应。

关键词: 眼镜蛇心脏毒素, 小鼠, 镇痛, 学习记忆, 眼镜蛇心脏毒素, 小鼠, 镇痛, 学习记忆

Abstract: To study the analgesic effect and ameliorating effect of cobra cardiotoxin (CTX) on learning and memory impairment induced by scopolamine in mice. The toxicity of CTX was measured in acute toxicity test. The analgesic effect of CTX in mice was measured by hot plate method and acetic aicd writhing test.
The ameliorating effect of CTX on learning and memory impairment induced by scopolamine in mice was studied by Y-Maze, Shuttle-box, Step-down and Water Maze methods respectively. LD50 value of CTX was 2.17 mg/kg. CTX ip 0.03 mg/kg, 0. O6mg/kg significantly prolonged the latency time of mice to hot plate stimuli, attenuated the writhing times induced by acetic aicd. CTX ip 0.06 mg/kg, 0.12mg/kg significantly reduced the errors of the mice in Y-Maze test, decreased the mices training times, shorten the escape latency in Shuttle box test. In Step-down test, CTX ip 0.06mg/kg, 0.12mg/kg increased the escape latency and reduced the number of errors, CTX also reduced the searching platform latency and swim path-length in Morris water maze test with mice. All of the effects are dose-dependent. The results above suggested that CTX has an effect of analgesia and is able to improve the learning and memory impairment induced by scopolarnine in mice.

Key words: mice, analgesia, learning and memory, cobra cardiotoxin, mice, analgesia, learning and memory

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