华东师范大学学报(自然科学版) ›› 2003, Vol. 2003 ›› Issue (1): 68-73.

• 论文 • 上一篇    下一篇

ED/Au/I-复合物修饰电极在细胞色素C测定中的应用

朱民；施国跃；刘敏；金利通   

  1. 华东师范大学 化学系， 上海 200062
  • 收稿日期:2002-01-25 修回日期:2002-10-13 出版日期:2003-03-25 发布日期:2003-03-25

Study of Gold Colloid Monolayer Modified Carbon Fiber Ultramicroelectrode and Its Application in Determination of Cytochrome C.

ZHU Min; SHI Guo-yue; LIU Min; JIN Li-tong   

  1. Department of Chemistry, East China Normal University, Shanghai 200062,China
  • Received:2002-01-25 Revised:2002-10-13 Online:2003-03-25 Published:2003-03-25

摘要： 该文报道了一种采用纳米材料制备修饰电极的新方法，制作了乙二胺/Au溶胶/I-（ED/Au/I-）复合物修饰的碳纤维微柱电极，并研究了该电极的化学行为。实验结果表明，该微电极对细胞色素C具有良好的催化还原特性，在6.5×10-7mol/L~1.2×10-5mol/L浓度范围内，还原峰电流与细胞色素C浓度呈现良好的线性，检测限为3.2×10-7mol/L；同时，该微电极具有选择性高，体积小和稳定性好等特点，适用于生命科学的研究，为细胞凋亡的病例、药理研究提供了新的数段和方法。

关键词: 金溶胶, 碳纤维修饰微电极, 细胞色素C, 金溶胶, 碳纤维修饰微电极, 细胞色素C

Abstract: A novel chemically modified ultramicroelectrode os described for the detection of cytochrome Cin this paper. Firstly, ethidene diamine(ED) was eletropolymerized on the surface of carbon fiber ultramicroelectrode(UME),and then gold colloid nano particles was deposited on the surface(Au/ED/UME). At last, we dipped the Au/ED/UME into the potassium iodide solution and then the iodide ion was absorbed onto the surface of Au/ED/UME. The electrochemical behavior of the prepared modified I-/Au/ED/UME was studied. The chemically modified UME, which was used as working electrode, has good electrocatal reduction of cytochrome c with a detection limit of 3.2×10-7mol/L and a linear concentration range of 6.5×10-7~1.2×10-5mol/L(vs.SCE). We think it has great potential for application for in vivo studying of cytochrome C.

Key words: Carbon fiber modified ultramicroelectrode, cytochrome C., Gold colloid nano particles, Carbon fiber modified ultramicroelectrode, cytochrome C.