华东师范大学学报(自然科学版) ›› 2010, Vol. 2010 ›› Issue (2): 134-140.

• 生命科学 • 上一篇    

一株产抗菌物质链霉菌的筛选及鉴定

汪学军1,2，闵长莉1,2，陈代杰3   

  1. 1. 皖西学院 化学与生命科学系,安徽 六安 237012； 2. 植物细胞工程安徽省工程技术研究中心,安徽 六安 237012；3. 上海医药工业研究院，上海 200040
  • 收稿日期:2009-03-18 修回日期:2009-05-30 出版日期:2010-03-25 发布日期:2010-03-25
  • 通讯作者: 汪学军

Isolation and identification of a strain of Streptomyces with antimicrobial activity

WANG Xue jun1,2,MIN Chang li1,2,CHEN Dai jie3   

  1. 1. Department of Chemistry and Life Science, West Anhui University, Liu’an Anhui 237012, China；2. Anhui Province Engineering Technology Research Center of Plant Cell Engineering, Liu’an Anhui237012, China；3. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China
  • Received:2009-03-18 Revised:2009-05-30 Online:2010-03-25 Published:2010-03-25
  • Contact: WANG Xue jun

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摘要： 从土壤中分离到一株对棉花黄萎病菌（Verticillium dahliae）有较强拮抗作用的链霉菌菌株HCCB10124，研究其拮抗性表明，对10种常见的病原真菌和部分细菌有拮抗作用，但对植物病原性真菌的抑制效果高于细菌.其形态特征、培养特性、生理生化特性、细胞壁组分与链霉菌属中的白网链霉菌（Streptomyces albireticuli）相同，16S rDNA序列的同源性达99%.根据多项分类原则和系统进化树的构建分析，将该菌株初步归属于白网链霉菌.

关键词: 白网链霉菌, 链霉菌HCCB10124, 抑菌活性, 筛选鉴定, 白网链霉菌, 链霉菌HCCB10124, 抑菌活性, 筛选鉴定

Abstract: A strain HCCB10124 was isolated from the soil against pathogenic fungus Verticillium dahliae.The growth of 10 plant pathogens and some bacteria were strongly inhibitded by strain HCCB10124 on PDA plate, but unconspicuous effects on bacteria comparatively. The morphology, cultural characteristics,physiological and biochemical properties ,chemotaxonomy and 16S rDNA sequences of this strain were studied. The results exhibited that the homology of 16S rDNA between Streptomyces strain HCCB10124 and Streptomyces albireticuli reached 99%. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the related species. According to the multiclassification principle and the phylogenetic analysis, the Streptomyces strain HCCB10124 should be nomenclatured as Streptomyces albireticuli.

Key words: Streptomyces strain HCCB10124, antimicrobial activity, screening and identification, Streptomyces albireticuli, Streptomyces strain HCCB10124, antimicrobial activity, screening and identification

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