华东师范大学学报(自然科学版) ›› 2010, Vol. 2010 ›› Issue (4): 85-91.

• 生命科学 • 上一篇    下一篇

利用两种方法筛选拟南芥 uro 突变体的回复子

张 亮， 杨 扬， 孙 越， 徐艳飞， 俞 琛，周 薇， 唐洪波， 李小方   

  1. 华东师范大学 生命科学学院，上海 200062
  • 收稿日期:2009-04-01 修回日期:2009-08-01 出版日期:2010-07-25 发布日期:2010-07-25
  • 通讯作者: 孙越

Screening the suppressors of uro by EMS mutagenesis and Ac/Dstransposon system

ZHANG Liang， YANG Yang， SUN Yue， XU Yanfei， YU Chen，ZHOU Wei， TANG Hongbo， LI Xiaofang   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2009-04-01 Revised:2009-08-01 Online:2010-07-25 Published:2010-07-25
  • Contact: SUN Yue

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摘要： 拟南芥URO基因与生长素的代谢密切相关，为了深入了解URO基因的作用机制及其所参与的遗传途径，以拟南芥叶发育异常的半显性突变体uro(upright rosette)为材料，利用EMS诱变和Ac/Ds转座子系统这两种方法筛选uro突变体的回复子.通过这两种方法获得了一些回复子，并对这些回复子进行了验证和初步分析，为今后的研究工作奠定了良好的基础.

关键词: 拟南芥, URO, EMS诱变, Ac/Ds转座子系统, 拟南芥, URO, EMS诱变, Ac/Ds转座子系统

Abstract: It was suggested that URO function might be associated with auxinmediated plant development. In order to know the role and the genetic net work ofURO gene, suppressors(double mutants with suppressed phenotype of uromutant) were screened through EMS mutagenesis and Ac /Ds transposon system. Several suppressors have been selected, validated and further analyzed. These suppressors will provide important clews for the function network of URO gene.

Key words: URO, EMS mutagenesis, Ac/Ds transposon system, Arabidopsis, URO, EMS mutagenesis, Ac/Ds transposon system

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