CE-LIF在氨基甲酸酯类农药研究中的应用

华东师范大学学报(自然科学版) ›› 2012, Vol. 2012 ›› Issue (5): 54-61.

CE-LIF在氨基甲酸酯类农药研究中的应用

杨玉林¹, 赵晓寅², 赵文杰², 周天舒²

1.通标标准技术服务（上海）有限公司，上海 200233; 2.华东师范大学环境科学系，上海 200062

收稿日期:2011-09-01 修回日期:2011-12-01 出版日期:2012-09-25 发布日期:2012-09-29

Study on carbamate pesticides by capillary electrophoresis with laser-induced fluorescence detection

YANG Yu-lin ¹, ZHAO Xiao-yin ², ZHAO Wen-jie ², ZHOU Tian-shu ²

1. SGS-CSTC Standards Technical Service (Shanghai), Ltd., Shanghai 200233, China;
2. Department of Environment Science, East China Normal University, Shanghai 200062, China

Received:2011-09-01 Revised:2011-12-01 Online:2012-09-25 Published:2012-09-29

摘要/Abstract

摘要： 用毛细管电泳激光诱导荧光检测法(CE-LIF)，对灭除威（XMC）、混杀威(2, 3, 5-trimethacarb)和灭虫威(mercaptodimethur)等3种氨基甲酸酯类农药进行了研究.在碱性条件下，氨基甲酸酯类农药水解产生甲胺并与荧光衍生试剂4-氯-7-硝基-2，1，3-苯并二唑(NBD-Cl)发生衍生化反应，产物经毛细管电泳激光诱导荧光检测法测定.对影响水解、衍生以及毛细电泳分离的因素如缓冲液酸度、浓度和衍生试剂的浓度等进行了详细讨论；在最佳条件下，3种农药的检出限分别可达3.0×10^-9、1.0×10^-9和3.0×10^-9 mol/L，并测定了它们的水解速率常数及活化能.该方法不仅可对氨基甲酸酯类农药进行测定，同时也可用于对这类农药的一些理化常数的测定.

关键词: 毛细管电泳, 激光诱导荧光, 氨基甲酸酯类农药, 水解速率常数, 活化能

Abstract: A sensitive and rapid capillary electrophoresis method with laser-induced fluorescence detection (CE-LIF) was developed for the determination of carbamate pesticides (including (3,5-dimethylphenyl) N-methylcarbamate (XMC), and mercaptodimethur). In alkaline solution, carbamate was hydrolyzed to methylamine, which can be derivatized by 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and detected by CE-LIF. Several parameters which influence the hydrolysis, derivatization and electrophoresis separation, such as pH, concentration of buffer solution and concentration of NBD-Cl, were investigated. The detection limits of the three carbamates were 3.0×10^-9、1.0×10^-9 and 3.0×10^-9 mol/L, respectively. The proposed method was successfully employed to determine the rate constants and activation energies for carbamates hydrolysis.

Key words: capillary electrophoresis, laser-induced fluorescence, carbamate pesticides, rate constant, activation energy

中图分类号:

Q657

杨玉林, 赵晓寅, 赵文杰周天舒. CE-LIF在氨基甲酸酯类农药研究中的应用[J]. 华东师范大学学报(自然科学版), 2012, 2012(5): 54-61.

YANG Yu-lin, ZHAO Xiao-yin, ZHAO Wen-jie, ZHOU Tian-shu. Study on carbamate pesticides by capillary electrophoresis with laser-induced fluorescence detection[J]. Journal of East China Normal University(Natural Sc, 2012, 2012(5): 54-61.

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