漂浮绿藻中缘管浒苔(Ulva linza)鉴定方法的探索

华东师范大学学报(自然科学版) ›› 2013, Vol. 2013 ›› Issue (5): 71-79.

漂浮绿藻中缘管浒苔(Ulva linza)鉴定方法的探索

陈斌斌¹, 马家海¹, SHIN Jong-Ahm², 高嵩¹

1. 上海海洋大学农业部水产种质资源发掘利用重点实验室, 上海 201306;
2. 全南大学校水产海洋大学養殖学科, 丽水市 556-901, 韩国

收稿日期:2012-09-01 修回日期:2012-12-01 出版日期:2013-09-25 发布日期:2013-09-30

An exploratory study on identification means Ulva linza from floating green algae

CHEN Bin-bin¹, MA Jia-hai¹, SHIN Jong-Ahm², GAO Song¹

1. Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecology Certificated by the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China 2. Aquaculture Program, College of Fisheries and Ocean Sciences, Chonnam National University, Yeosu 550-749, Korea

Received:2012-09-01 Revised:2012-12-01 Online:2013-09-25 Published:2013-09-30

摘要/Abstract

摘要： 以研究漂浮绿藻中的缘管浒苔(\textit{Ulva linza})为出发点,
根据绿藻的形态学特征, 选取漂浮绿藻中具有\textit{U.
linza}重要特征的藻体进行形态学、分子生物学的分类学研究. 结果显示,
具有\textit{U. linza}重要特征的漂浮藻体大多为LPP复合体(\textit{U.
linza-procera-prolifera})类群(15个样品),
而且采自江苏的DF05、\!山东的QD03以及浙江的XS01、XS02等4个固着类型的样品也在该分支中.
\textit{Ulva flexuosa}分支中有2个样品(均为采自福建的固着种类),
\textit{Ulva compressa}分枝包含了如东的4个漂浮样品.
采自如东内河、射阳沿海池塘的漂浮藻体也均被包含在LPP复合体中.
25个样品中只有4个被鉴定为\textit{U. linza},
与其形态特征研究结果相符合.
漂浮绿藻的形态学特征与分子生物学研究结果存在很大差别.
这说明单纯地依靠传统分类学特征或分子生物学手段对漂浮绿藻中的缘管浒苔(\textit{U.
linza})进行分类是不可取的, 甚至对漂浮绿藻都不能适用.
在同一地点采集到的漂浮绿藻中, 大都存在不同形态特征的类型,
经过分子生物学分析后被鉴定为不同的种类.
根据ITS序列的系统进化树分析结果,
绿潮藻中至少存在LPP复合体中的种类以及\textit{U. compressa}.

关键词: 绿潮, 缘管浒苔, 形态学, ITS, 分子生物学

Abstract: To study on morphological characteristics of \textit{Ulva
linza} from floating green algae, we selected specimens with the
typical features of \textit{U. linza. }Only 4 specimens from 25
samples were determined as \textit{U. linza }from the morphological
characteristics results. The molecular biology research by means of
nuclear-encoded ITS sequences analysis was also carried out. Fifteen
floating specimens with characters of \textit{U. linza} clustered
into the LPP (\textit{U. linza-procera-prolifera}) complex group
including the attached species DF05 from Jiangsu Province, QD03 from
Shandong Province and XS01, XS02 from Zhejiang Province. The
floating green algae from Rudong inland river and coastal pool of
Sheyang were included in LPP complex as well. \textit{U. flexuosa}
group included two specimens that just were fixation growth algae
from Fujian Province. \textit{U. compressa} group had four
specimens, and they all were floating green algae. A great disparity
existed between the morphological characteristics and the molecular
results. Identification of \textit{U. linza} and other floating
green algae based on either morphology or ITS sequence unilaterally
was not reliable. Almost all of floating green algae from the same
location had certain morphological types, and they were considered
to be different species by the ITS sequences analysis. The result of
N-J tree and ITS data showed that the species in LPP complex and
\textit{U. compressa} existed in the green tide. But\textit{ U.
flexuosa }did not exist in the floating green algae in this
research.

Key words: green tide, Ulva linza, morphology, ITS, molecular biology

中图分类号:

陈斌斌, 马家海, SHIN Jong-Ahm, 高嵩. 漂浮绿藻中缘管浒苔(Ulva linza)鉴定方法的探索[J]. 华东师范大学学报(自然科学版), 2013, 2013(5): 71-79.

CHEN Bin-bin, MA Jia-hai, SHIN Jong-Ahm, GAO Song. An exploratory study on identification means Ulva linza from floating green algae[J]. Journal of East China Normal University(Natural Sc, 2013, 2013(5): 71-79.

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