华东师范大学学报(自然科学版) ›› 2013, Vol. 2013 ›› Issue (5): 61-70, 79.

• 生命科学 • 上一篇    下一篇

PC交联脱细胞牛心包膜的力学性能、稳定性和血液相容性

马 兵1,2, 翟万银2, 张嘉敏3, 孙晓宁4, 朱自严3, 张红锋1, 常 江2   

  1. 1.华东师范大学 生命科学学院，上海 200062；
    2.中国科学院上海硅酸盐研究所 生物材料与组织工程中心，上海 200050；
    3.上海市血液中心 上海市输血研究所，上海 200051；
    4.复旦大学附属中山医院 心脏外科，上海 200032
  • 收稿日期:2012-07-01 修回日期:2012-10-01 出版日期:2013-09-25 发布日期:2013-09-30

Crosslinking of decellularized bovine pericardium matrix by procyanidins: mechanical properties, stability and hemo-compatibility

MA Bing1,2, ZHAI Wan-yin2, ZHANG Jia-min3, SUN Xiao-ning4, ZHU Zi-yan3, ZHANG Hong-feng1, CHANG Jiang2   

  1. 1. School of Life Sciences, East China Normal University, Shanghai 200062, China;
    2. Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China;
    3. Shanghai Blood Center, Shanghai Institute of Blood Transfusion, Shanghai 200051, China;
    4. Department of Cardiac Surgery, Zhongshan Hospital Fudan University, Shanghai 200032, China
  • Received:2012-07-01 Revised:2012-10-01 Online:2013-09-25 Published:2013-09-30

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摘要： 测定了原花青素（PC）交联的脱细胞牛心包膜（dbpECM）的力学性能、稳定性和血液相容性.实验结果显示，2.5 mg/mL原花青素交联能将拉伸强度从29.1 MPa提高到51.3 MPa，并保持弹性模量基本不变，而抗胶原酶降解能力与戊二醛相当；PC-dbpECM浸泡于D-Hanks溶液中，仅过量未交联原花青素在一周内释放，而交联的PC在PC-dbpECM中保持稳定.1 mg/mL PC即可基本抑制体外钙化.PC或GA交联的dbpECM都有较好的血液相容性，但PC交联明显优于GA. 2.5 mg/mL PC交联的dbpECM血小板的粘附率（7.8%）显著低于未交联组（26.1%）和GA交联组（36.6%），表明PC-dbpECM具有较好的抗血栓形成能力.结果表明PC-dbpECM具有良好的力学性能，稳定性和血液相容性，有可能成为制备人工心脏瓣膜的优良材料.

关键词: 原花青素, 脱细胞牛心包膜, 力学性能, 稳定性, 血液相容性

Abstract: In this study, decellularized bovine pericardium matrix (dbpECM) was cross-linked by using PC at series concentrations. Mechanical properties, stability and hemo-compatibility of PC-crosslinked dbpECM (PC-dbpECM) were investigated. The results show that the tensile strength of the PC-dbpECM which crosslinked by the 2.5 mg/mL PC is increased from 29.1 MPa to 51.3 MPa with the elastic modulus roughly at basic level. Resistance of PC-dbpECM to collagenase hydrolysis is equivalent to that of GA-crosslinked. When PC-dbpECM soaked in D-Hanks solution, only the excessive non-crosslinked PC released within a week, leaving the crosslinked PC stable all the experiment period. PC crosslinked dbpECM (1 mg/mL) can effectively inhibit calcification in vitro. Both PC- and GA-dbpECM have ideal hemo-compatibility, but the PC cross-linking is superior to that of GA. The rate of platelet adhesion on the surface of 2.5 mg/mL PC crosslinked dbpECM (7.8%) was significantly lower than that of non-crosslinked group (26.1%) and the GA crosslinked group (36.6%), indicating that PC-dbpECM has low coagulation spotential. These data suggest that PC-dbpECM has good mechanical properties, stability and hemo-compatibility, suitable for preparing biological heart valves.

Key words: procyanidins, decellularized bovine pericardium, mechanical properties, stability, hemo-compatibility

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