月季切花瓶插期间花瓣糖代谢及其相关酶活性的研究

华东师范大学学报(自然科学版) ›› 2013, Vol. 2013 ›› Issue (6): 159-164.

月季切花瓶插期间花瓣糖代谢及其相关酶活性的研究

李龙^1,2, 王海宏², 陈召亮², 乔勇进², 王利¹

1. 山东农业大学林学院，山东泰安 271018；
2. 上海市农业科学院农产品保鲜加工研究中心，上海 201403

收稿日期:2012-12-01 修回日期:2013-03-01 出版日期:2013-11-25 发布日期:2014-01-13

Analysis of sugar metabolism and its related enzymes activities during vase-holding days of cut rose petal

LI Long^1,2, WANG Hai-hong², CHEN Zhao-liang², QIAO Yong-jin², WANG Li¹

1. College of Forestry, Shandong Agricultural University, Taian Shandong 271018, China;
2. Agricultural Products Storage & Processing Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received:2012-12-01 Revised:2013-03-01 Online:2013-11-25 Published:2014-01-13

摘要/Abstract

摘要： 为探究糖代谢及其酶对月季脱离母体后的生命活动的影响，以月季（Rosa hybrida Hort）切花“黑魔术”（cv. Black Magic flower）和“影星”（cv. Movie Star flower）为试验材料，分析瓶插期间花瓣糖代谢及其相关酶活性的变化.试验表明：随着月季切花瓶插时间的延长和花瓣开放，花瓣中葡萄糖（G）和果糖（F）含量显著增加，蔗糖含量随之降低，切花“影星”的己糖（G+F）含量、花径的增长和花朵鲜重都明显高于“黑魔术”；花瓣中酸性转化酶（AI）活性与中性转化酶（NI）、蔗糖合成酶（SS）和磷酸蔗糖合成酶（SPS）相比具有较高的水平，AI活性与蔗糖降解指数（SDI）成正相关，AI是降解蔗糖并积累己糖的关键酶，而花瓣中己糖积累在月季切花生长发育过程中起重要作用.

关键词: 月季切花花瓣, 糖代谢, 糖代谢酶活

Abstract: Analyze changes of sugar metabolism and its related enzymes activities during vase-holding days of cut roses named “cv. Black Magic flower” and “cv. Movie Star flower” were studied. The results showed that glucose and fructose contents increased rapidly with the cell enlargement in the petals and during flower growth, but the content of sucrose dropped gradually. The cut rose named “cv. Movie Star flower” had a larger hexoses (glucose+fructose) content , fresh weight and a bigger flower diameter fresh weight than “cv. Black Magic flower” . The activity of acid invertase (AI) was always at a higher level than those of the neutral invertase (NI), sucrose synthase (SS) and sucrose phosphate synthase (SPS). The activity of acid invertase (AI) was positively correlated with sucrose degradation index (SDI). In conclusion, the acid invertase (AI) was a key enzyme in the sucrose degradation and hexoses accumulation, while hexose accumulation in the petal might be important for flower growth and development in cut rose.

Key words: cut roses petals, sugar metabolism, enzymes activities

中图分类号:

李龙, 王海宏, 陈召亮, 乔勇进, 王利. 月季切花瓶插期间花瓣糖代谢及其相关酶活性的研究[J]. 华东师范大学学报(自然科学版), 2013, 2013(6): 159-164.

LI Long, WANG Hai-hong, CHEN Zhao-liang, QIAO Yong-jin, WANG Li. Analysis of sugar metabolism and its related enzymes activities during vase-holding days of cut rose petal[J]. Journal of East China Normal University(Natural Sc, 2013, 2013(6): 159-164.

参考文献

［1］彭彪,彭东辉,许贤书.月季切花保鲜技术研究进展［J］.林业勘察设计，2007(2)：82-87.

［2］秦红玫.月季切花衰老机理研究进展［J］.安徽农业科学, 2006，34(5)：841-843.

［3］陈林.国际玫瑰切花市场调查与分析［J］.温室园艺, 2005(11)：22-24.

［4］ FISHER D B, WANG N. Sucrose concentration gradients along the post-phloem transport pathway in the maternal tissues of developing wheat grains［J］. Plant Physiol, 1995，109：587-592.

［5］ WANG H L，LEE P D，CHEN W L，et al. Osmotic stress-induced changes of sucrose metabolism in cultured sweet potato cells［J］. Journal of Experimental Botany, 2000，51：1991-1999.

［6］刘凌霄，沈法富，卢合全,等.蔗糖代谢中蔗糖磷酸合成酶(SPS)的研究进展［J］.分子植物育种，2005, 3(2)；275-281.

［7］刘洋，林希昊，姚艳丽,等. 高等植物蔗糖代谢研究进展［J］.中国农业通报，2012，28(6)：145-152.

［8］ SOOD S, VYAS D, NAGAR K. Physiological and biochemical studies during flower development in two rose species. Scientia Horticulturae，2006，108：390-396.

［9］甘彩霞，吴楚. 蔗糖代谢中3类酶的研究进展［J］.长江大学学报（自然科学版）农业卷，2007，4(1)：74-78. 

［10］ STURM A. Invertases primary structures, functions, and roles in plant development and sucrose partitioning［J］. Plant Physiology，1999，121：1-7.

［11］张明方，李志凌.高等植物中与蔗糖代谢相关的酶［J］.植物生理学通讯，2002，38(3)：289-295.

［12］胡志群，王惠聪，胡桂兵.高效液相色谱测定荔枝果肉中的糖、酸和维生素C［J］.果树学报，2005，22(5)：582-585.

［13］孟海玲，王有年，李奕松,等.桃树子房发育初期蔗糖含量及其相关酶活性的变化［J］.中国农业科学,2008，41(3)：779-785.

［14］史国安，郭香凤，张国海，等.牡丹开花和衰老期间花瓣糖代谢的研究［J］.园艺学报，2009，36(8)：1184-1190.

［15］ KING A D, HO L C, BAKER D A. Activity of sucrose hydrolysing enzymes and sugar accumulation during tomato fruit development ［J］. Plant Growth Regulation，1997，22：193-201.

［16］ KELLER F, LUDLOW M M. Carbohydrate metabolism in drought-stressed leaves of pigeonpea (Cajanus cajan) ［J］. Journal of Experimental Botany，1993，44：1351-1359.

［17］ RANWALA A P, IWANAMI S S, MASUDA H. Acid and neutral invertases in the mesocarp of developing muskmelon (CucumismeloL. cvPrince) fruit ［J］. Plant Physiology，1991，96：881-886.

［18］ RUFTY T W, KERR P S, HUBER S C. Characterization of diurnal changes in activities of enzymes involved in sucrose biosynthesis［J］. Plant Physiology，1983，73：428-433.

［19］ VAN DOORN W G, VAN MEETEREN U. Flower opening and closure: a review ［J］. Journal of Experimental Botany，2003，54：1801-1812.

［20］ HAMMOND J B. Changes in amylase activity during rose bud opening ［J］. Scientia Horticulturae，1982，16： 283-289.

［21］ BIELESKI R L. Fructan hydrolysis drives petal expansion in the ephemeral daylily flower ［J］. Plant Physiology，1993，103：213-219.

［22］ YAMADA K, ITO M, OYAMA T, et al. Analysis of sucrose metabolism during petal growth of cut roses ［J］. Postharvest Biology and Technology，2007，43：174-177.

［23］ KUIPER D, RIBOT S, VAN REENEN H S, et al. The effect of sucrose on the flower bud opening of ‘Madelon’ cut rose ［J］. Scientia Horticulturae，1995，60：325-336.