姜黄素对老年鼠脑内氧化性平衡和星形胶质细胞的影响

doi:10.3969/j.issn.1000-5641.2015.06.014

华东师范大学学报(自然科学版) ›› 2015, Vol. 2015 ›› Issue (6): 108-116.doi: 10.3969/j.issn.1000-5641.2015.06.014

姜黄素对老年鼠脑内氧化性平衡和星形胶质细胞的影响

张盼，俞彬，刘莎，段雅乐，赵政

华东师范大学脑功能基因组学教育部重点实验室，上海市脑功能基因组学重点实验室，神经药理研究室，上海200062

收稿日期:2014-09-12 出版日期:2015-11-25 发布日期:2015-12-23
通讯作者: 赵政，男，研究员，研究方向为神经生物学. E-mail: zzhao@braim.ecnu.edu.cn
作者简介:张盼，女，博士研究生，研究方向为药理学.Email: 478664205@qq.com.
基金资助:
国家自然科学基金（31171019，31200820）；脑功能基因组学教育部重点实验室，上海市脑功能基因组学重点实验室（华东师范大学）开放项目基金

Effects of curcumin on oxidative stress status and thefunctions of astrocytes in the brain of aged rats

ZHANG Pan, YU Bin, LIU Sha, DUAN Ya-Le, ZHAO Zheng

Received:2014-09-12 Online:2015-11-25 Published:2015-12-23

摘要/Abstract

摘要： 以SD大鼠为动物模型，采用Western blotting、酶活性测定、免疫荧光和高效液相色谱质谱（LCMS）方法考察姜黄素处理12周后正常衰老大鼠皮层和海马的氧化性平衡和星形胶质细胞形态功能的变化.结果显示，姜黄素降低了老年鼠脑内氧化产物MDA和8OHdG的含量，提升了γ谷氨酰胺半胱氨酸合成酶（GCS）的活性并且促进GSH的产生，提示姜黄素可显著改善伴随正常衰老过程的脑内氧化性平衡；同时姜黄素可以抑制胶质纤维酸性蛋白（GFAP）的过度表达，提升GS活性，增加GDNF水平和D丝氨酸含量，表明其抑制星形胶质细胞过度活化的同时也改善了星形胶质细胞的功能.

关键词: 姜黄素, 衰老, 氧化应激, 星形胶质细胞

Abstract: The change of oxidative balance and astrocyte in the cortex and hippocampus of aged SD rats were investigated by Western blotting, enzyme assay, immunofluorescence and HPLCMS. The data showed that curcumin reduced the level of MDA and 8OHdG and enhanced the level of GSH and GCS activity, which indicated that curcumin inhibited the oxidative damage in the brian; the detection of the level of glial fibrillary acidic protein (GFAP) indicated curcumin inhibited the excessive activation of astrocytes and also the results of the activity of GS, the level of GDNF and Dserine showed that curcumin improved the function of astrocytes.

Key words: curcumin, aging, oxidative stress, astrocyte

中图分类号:

Ｑ１８９

张盼, 俞彬, 刘莎, 段雅乐, 赵政. 姜黄素对老年鼠脑内氧化性平衡和星形胶质细胞的影响[J]. 华东师范大学学报(自然科学版), 2015, 2015(6): 108-116.

ZHANG Pan, YU Bin, LIU Sha, DUAN Ya-Le, ZHAO Zheng. Effects of curcumin on oxidative stress status and thefunctions of astrocytes in the brain of aged rats[J]. Journal of East China Normal University(Natural Sc, 2015, 2015(6): 108-116.

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姜黄素对老年鼠脑内氧化性平衡和星形胶质细胞的影响

Effects of curcumin on oxidative stress status and thefunctions of astrocytes in the brain of aged rats

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