华东师范大学学报（自然科学版） >

2024 , Vol. 2024 >Issue 6: 151 - 160

DOI: https://doi.org/10.3969/j.issn.1000-5641.2024.06.013

生态风险与管控

抗生素与抗性基因在畜禽养殖中的环境影响及生态风险

  • 胡雯桉 ,
  • 沈城 ,
  • 孙慧伦 ,
  • 王强 ,
  • 刘敏
展开
  • 1. 华东师范大学 生态与环境科学学院, 上海　200241
    2. 上海市环境科学研究院, 上海　200233
    3. 华东理工大学 资源与环境工程学院, 上海　200237
    4. 华东师范大学 地理科学学院, 上海　200241
王　强, 男, 高级工程师, 硕士生导师, 研究方向为生态学. E-mail: wqflora@163.com

收稿日期: 2024-09-18

  录用日期: 2024-11-07

  网络出版日期: 2024-11-29

基金资助

上海市科委项目 (19ZR1444100)

收起

Environmental impacts and ecological risks of antibiotics and resistance genes in livestock and poultry farming

  • Wenan HU ,
  • Cheng SHEN ,
  • Huilun SUN ,
  • Qiang WANG ,
  • Min LIU
Expand
  • 1. School of Ecological and Environmental Sciences, East China Normal University, Shanghai　200241, China
    2. Shanghai Academy of Environmental Sciences, Shanghai　200233, China
    3. School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai　200237, China
    4. School of Geographic Sciences, East China Normal University, Shanghai　200241, China

Received date: 2024-09-18

  Accepted date: 2024-11-07

  Online published: 2024-11-29

Fold

摘要

对畜禽养殖环境中抗生素与抗性基因的影响与潜在的生态风险进行了综述, 通过对相关文献进行系统调研总结了不同地区畜禽养殖业中抗生素的使用情况. 研究发现, 畜禽粪便及粪肥还田后土壤中抗生素与抗性基因残留浓度水平因粪肥种类、地区、养殖场规模和管理措施等因素而存在差异; 同时, 抗生素和抗性基因在土壤中的迁移转化受土壤类型及施肥方式的影响. 此外, 本研究特别探讨了抗性基因在土壤中通过水平基因扩散等机制传播对土壤微生物的影响, 系统阐述了抗生素与抗性基因的生态风险. 最后, 结合畜禽粪肥还田的污染现状, 对未来减少环境中抗生素及抗性基因积累和扩散进行了总结与展望, 期望为全面了解抗生素及抗性基因在畜禽养殖中的环境影响和生态风险提供有力支持.

关键词： 抗生素; 抗性基因; 畜禽粪便; 生态风险

本文引用格式

胡雯桉 , 沈城 , 孙慧伦 , 王强 , 刘敏 . 抗生素与抗性基因在畜禽养殖中的环境影响及生态风险[J]. 华东师范大学学报（自然科学版）, 2024 , 2024(6) : 151 -160 . DOI: 10.3969/j.issn.1000-5641.2024.06.013

Abstract

This article provides a review of the impacts and potential ecological risks of antibiotics and resistance genes in livestock and poultry farming environments. Through systematic research on the relevant literature, the use of antibiotics in livestock and poultry farming in different regions was summarized. The study found that the residual concentrations of antibiotics and resistance genes in soil after returning livestock and poultry manure to the field varied due to factors such as manure type, region, farm scale, and management measures. Moreover, the migration and transformation of antibiotics and resistance genes in soil are influenced by the soil type and fertilization methods. This study specifically explored the impact of the spreading of resistance genes through mechanisms such as horizontal gene diffusion by soil microorganisms and systematically elucidated the ecological risks of antibiotics and resistance genes. Finally, based on the current pollution situation of returning livestock manure to the field, a summary and outlook on reducing the accumulation and diffusion of antibiotics and resistance genes in the environment were prepared. The work is expected to provide strong support for a comprehensive understanding of the environmental impacts and ecological risks of antibiotics and resistance genes in livestock and poultry farming.

Key words： antibiotic; antibiotic resistance gene; livestock manure; ecological risk

参考文献

1 张瑞增, 孟婷. 21世纪以来我国畜禽粪污资源化利用的研究进展及趋势——基于CNKI和CiteSpace的可视化分析[J]. 中国农业资源与区划, 2024, 44: 1-16.
2 LI Y L, LI Q J, JI Z Y, et al.. Current status and spatiotemporal evolution of antibiotic residues in livestock and poultry manure in China. Agriculture, 2023, 13 (10): 1877.
3 ZHOU X, WANG J, LU C, et al.. Antibiotics in animal manure and manure-based fertilizers: Occurrence and ecological risk assessment. Chemosphere, 2020, 255 (C): 127006.
4 ZHA Y, LI Q H, LIU H, et al.. Occurrence and ecological risk assessment of antibiotics in manure and the surrounding soil from typical chicken farms in Hangzhou, China. Frontiers in Environmental Science, 2023, 11, 1241405.
5 MENZ J, OLSSON O, KüMMERER K.. Antibiotic residues in livestock manure: Does the EU risk assessment sufficiently protect against microbial toxicity and selection of resistant bacteria in the environment?. Journal of Hazardous Materials, 2019, 379, 120807.
6 XU L S, WANG W Z, XU W H.. Effects of tetracycline antibiotics in chicken manure on soil microbes and antibiotic resistance genes (ARGs). Environmental Geochemistry and Health, 2021, 44 (1): 1- 12.
7 GABRIELA M L, MIHAELA J, CARMEN P, et al.. Insights into the impact of manure on the environmental antibiotic residues and resistance pool. Frontiers in Microbiology, 2022, 13, 965132- 965132.
8 MARTINEZ J L.. Environmental pollution by antibiotics and by antibiotic resistance determinants. Environmental Pollution, 2009, 157 (11): 2893- 2902.
9 SARMAH A K, MEYER M T, BOXALL A B A.. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere, 2006, 65 (5): 725- 759.
10 CASTANON J I R.. History of the use of antibiotic as growth promoters in European poultry feeds. Poultry Science, 2007, 86 (11): 2466- 2471.
11 ZHANG Q Q, YING G G, PAN C G, et al.. Comprehensive evaluation of antibiotics emission and fate in the river basins of China: Source analysis, multimedia modeling, and linkage to bacterial resistance. Environmental Science & Technology, 2015, 49 (11): 6772- 6782.
12 ZHOU B R, WANG C, ZHAO Q, et al.. Prevalence and dissemination of antibiotic resistance genes and coselection of heavy metals in Chinese dairy farms. Journal of Hazardous Materials, 2016, 320, 10- 17.
13 BOECKEL T P V, GLENNON E E, CHEN D, et al.. Reducing antimicrobial use in food animals. Science, 2017, 357 (6358): 1350- 1352.
14 KUPPUSAMY S, VENKATESWARLU K, MEGHARAJ M.. Tetracycline and fluoroquinolone antibiotics contamination in agricultural soils fertilized long-term with chicken litter: Trends and ravages. Science of the Total Environment, 2024, 946, 174286.
15 MU Q H, LI J, SUN Y X, et al.. Occurrence of sulfonamide-, tetracycline-, plasmid-mediated quinolone- and macrolide-resistance genes in livestock feedlots in Northern China. Environmental Science and Pollution Research International, 2015, 22 (9): 6932- 6940.
16 凌文翠, 范玉梅, 方瑶瑶, 等.. 京津冀地区畜禽养殖业抗生素污染现状分析. 环境工程技术学报, 2018, 8 (4): 390- 397.
17 ADEGBEYE M J, ADETUYI B O, IGIRIGI A I, et al.. Comprehensive insights into antibiotic residues in livestock products: Distribution, factors, challenges, opportunities, and implications for food safety and public health. Food Control, 2024, 163, 110545.
18 LI C, CHEN J Y, WANG J H, et al.. Occurrence of antibiotics in soils and manures from greenhouse vegetable production bases of Beijing, China and an associated risk assessment. Science of the Total Environment, 2015, 521/522, 101- 107.
19 CONDE-CID M, FERREIRA-COELHO G, FERNáNDEZ-CALVI?O D, et al.. Single and simultaneous adsorption of three sulfonamides in agricultural soils: Effects of pH and organic matter content. Science of the Total Environment, 2020, 744, 140872- 140872.
20 李晓华. 规模化猪场粪污中典型抗生素归趋行为及抗性基因扩散特征研究 [D]. 北京: 中国农业科学院, 2018.
21 ZHAN L, XIA Z W, XU Z M.. Thermal desorption behavior of fluoroquinolones in contaminated soil of livestock and poultry breeding. Environmental Research, 2022, 211, 113101- 113101.
22 DU D L, ZHOU J, ZHANG K Q, et al.. Seasonal pollution characteristics of antibiotics on pig farms of different scales. International Journal of Environmental Research and Public Health, 2022, 19 (14): 8264- 8264.
23 OBEROI A S, JIA Y Y, ZHANG H Q, et al.. Insights into the fate and removal of antibiotics in engineered biological treatment systems: A critical review. Environmental Science and Technology, 2019, 53 (13): 7234- 7264.
24 QIAO M, YING G G, SINGER A C, et al.. Review of antibiotic resistance in China and its environment. Environment International, 2018, 110, 160- 172.
25 GU D, FENG Q, GUO C, et al.. Occurrence and risk assessment of antibiotics in manure, soil, wastewater, groundwater from livestock and poultry farms in Xuzhou, China. Bulletin of Environmental Contamination and Toxicology, 2019, 103 (4): 590- 596.
26 张卫艺, 曹子薇, 直俊强, 等.. 北京规模化养殖场畜禽粪便中养分、重金属和抗生素含量分析. 畜牧与兽医, 2023, 55 (4): 41- 49.
27 张慧敏, 章明奎, 顾国平.. 浙北地区畜禽粪便和农田土壤中四环素类抗生素残留. 生态与农村环境学报, 2008, (3): 69- 73.
28 PENG S, ZHANG H Y, SONG D, et al.. Distribution of antibiotic, heavy metals and antibiotic resistance genes in livestock and poultry feces from different scale of farms in Ningxia, China. Journal of Hazardous Materials, 2022, 440, 129719- 129719.
29 彭秋, 王卫中, 徐卫红.. 重庆市畜禽粪便及菜田土壤中四环素类抗生素生态风险评价. 环境科学, 2020, 41 (10): 4757- 4766.
30 AARESTRUP F M.. The livestock reservoir for antimicrobial resistance: A personal view on changing patterns of risks, effects of interventions and the way forward. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2015, 370 (1670): 20140085.
31 CHEE-SANFORD J C, MACKIE R I, KOIKE S, et al.. Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste. Journal of Environmental Quality, 2009, 38 (3): 1086- 1108.
32 DAI S T, LIU D J, HAN Z M, et al.. Mobile tigecycline resistance gene tet(X4) persists with different animal manure composting treatments and fertilizer receiving soils. Chemosphere, 2022, 307 (P3): 135866- 135866.
33 HE L Y, LIU Y S, SU H C, et al.. Dissemination of antibiotic resistance genes in representative broiler feedlots environments: Identification of indicator ARGs and correlations with environmental variables. Environmental Science and Technology, 2014, 48 (22): 13120- 13129.
34 邹威, 金彩霞, 魏闪, 等.. 华北地区不同规模畜禽养殖场粪便中抗生素抗性基因污染特征. 农业环境科学学报, 2020, 39 (11): 2640- 2652.
35 PAN Y, ZENG J X, ZHANG L X, et al.. The fate of antibiotics and antibiotic resistance genes in large-scale chicken farm environments: Preliminary view of the performance of national veterinary antimicrobial use reduction action in Guangdong, China. Environment International, 2024, 191, 108974.
36 CHENG W X, CHEN H, SU C, et al.. Abundance and persistence of antibiotic resistance genes in livestock farms: A comprehensive investigation in eastern China. Environment International, 2013, 61, 1- 7.
37 GUO T, LOU C L, ZHAI W W, et al.. Increased occurrence of heavy metals, antibiotics and resistance genes in surface soil after long-term application of manure. Science of the Total Environment, 2018, 635, 995- 1003.
38 HU X G, ZHOU Q X, LUO Y.. Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, northern China. Environmental Pollution, 2010, 158 (9): 2992- 2998.
39 LI Y W, WU X L, MO C H, et al.. Investigation of sulfonamide, tetracycline, and quinolone antibiotics in vegetable farmland soil in the Pearl River Delta area, southern China. Journal of Agricultural and Food Chemistry, 2011, 59 (13): 7268- 7276.
40 许静, 王娜, 孔德洋, 等.. 有机肥源磺胺类抗生素在土壤中的降解规律及影响因素分析. 环境科学学报, 2015, 35 (2): 550- 556.
41 张健, 关连珠, 颜丽.. 鸡粪中3种四环素类抗生素在棕壤中的动态变化及原因分析. 环境科学学报, 2011, 31 (5): 1039- 1044.
42 方镜人, 母悦, 唐柏彬, 等. 三峡库区典型农业小流域土壤中抗生素的时空分布与生态风险[J]. 环境科学, 2024, 45(11): 1-15.
43 ZHU Y, JOHNSON T A, SU J Q, et al.. Diverse and abundant antibiotic resistance genes in Chinese swine farms. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 (9): 3435- 3440.
44 WELLINGTON E M, BOXALL A B, CROSS P, et al.. The role of the natural environment in the emergence of antibiotic resistance in Gram-negative bacteria. The Lancet Infectious Diseases, 2013, 13 (2): 155- 165.
45 SARANYA K, KADIYALA V, MALLAVARAPU M, et al.. Contamination of long-term manure-fertilized Indian paddy soils with veterinary antibiotics: Impact on bacterial communities and antibiotics resistance genes. Applied Soil Ecology, 2023, 192, 105106.
46 程建华, 唐翔宇, 刘琛.. 紫色土丘陵区畜禽养殖场土壤中抗生素抗性基因分布特征. 环境科学, 2019, 40 (7): 3257- 3262.
47 张俊华, 陈睿华, 刘吉利, 等.. 宁夏养牛场粪污和周边土壤中抗生素及抗生素抗性基因分布特征. 环境科学, 2021, 42 (6): 2981- 2991.
48 张毓森, 叶军, 苏建强.. 粪肥与铜一次性施用对农田土壤抗生素抗性基因的长期影响. 应用与环境生物学报, 2019, 25 (2): 328- 332.
49 HUANG X, LIU C X, LI K, et al.. Occurrence and distribution of veterinary antibiotics and tetracycline resistance genes in farmland soils around swine feedlots in Fujian Province, China. Environmental Science and Pollution Research International, 2013, 20 (12): 9066- 9074.
50 王经邦, 王亚菲, 姚燕来, 等. 超高温堆肥在有机固体废弃物抗生素抗性基因等生物污染消减中的研究进展[J]. 浙江农业科学, 2024, 64: 1-7.
51 QIAN X, SUN W, GU J, et al.. Reducing antibiotic resistance genes, integrons, and pathogens in dairy manure by continuous thermophilic composting. Bioresource Technology, 2016, 220, 425- 432.
52 LIAO H P, LU X M, RENSING C, et al.. Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge. Environmental Science and Technology, 2018, 52 (1): 266- 276.
53 余震, 周顺桂.. 超高温好氧发酵技术: 堆肥快速腐熟与污染控制机制. 南京农业大学学报, 2020, 43 (5): 781- 789.
54 郑宁国, 黄南, 王卫卫, 等.. 高温堆肥过程对猪粪来源抗生素抗性基因的影响. 环境科学, 2016, 37 (5): 1986- 1992.
55 李娟, 魏源送, 陈倩.. 北京地区畜禽养殖场周边蔬菜地生食蔬菜抗生素耐药基因(ARGs)的赋存特性. 环境化学, 2018, 37 (4): 625- 635.
56 ALLEN H K, DONATO J, WANG H H, et al.. Call of the wild: Antibiotic resistance genes in natural environments. Nature Reviews Microbiology, 2010, 8 (4): 251- 259.
57 方淑霞, 王大力, 朱丽华, 等.. 抗生素对微生物的联合与低剂量毒性研究进展. 生态毒理学报, 2015, 10 (2): 69- 75.
58 牛明芬, 谢田宾, 王颜红, 等.. 畜禽粪肥中抗生素残留对土壤微生物及抗性基因的影响分析研究进展. 环境污染与防治, 2024, 46 (2): 252- 261.
59 GUO Z B, WAN S X, HUA K K, et al.. Fertilization regime has a greater effect on soil microbial community structure than crop rotation and growth stage in an agroecosystem. Applied Soil Ecology, 2020, 149, 103510- 103510.
60 李小嘉, 李江涛, 倪杰, 等.. 集约化畜禽粪便施用对土壤微生物群落功能多样性的影响. 广东农业科学, 2014, 41 (6): 193- 198.
61 CHEN G L, YUAN J H, CHEN H, et al.. Animal manures promoted soil phosphorus transformation via affecting soil microbial community in paddy soil. Science of the Total Environment, 2022, 831, 154917- 154917.
62 林曼霞. 室内粪土模型中土霉素胁迫下反硝化菌群分子响应特征研究 [D]. 广州: 华南农业大学, 2018.
63 易良银, 梁玉婷, 赵慧慧, 等.. 土壤中抗性基因的产生, 扩散传播以及消减的研究进展. 现代生物医学进展, 2015, 15 (9): 1752- 1759.
64 ZHAO Y, COCERVA T, COX S, et al.. Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils. Science of the Total Environment, 2019, 656, 512- 520.
65 QIAN X, GU J, SUN W, et al.. Diversity, abundance, and persistence of antibiotic resistance genes in various types of animal manure following industrial composting. Journal of Hazardous Materials, 2018, 344, 716- 722.
66 沈洪艳, 胡小敏.. 不同环境介质中污染物生态风险评价方法的国内研究进展. 河北科技大学学报, 2018, 39 (2): 176- 182.
67 ZHANG H B, LUO Y M, WU L H, et al.. Residues and potential ecological risks of veterinary antibiotics in manures and composts associated with protected vegetable farming. Environmental Science and Pollution Research International, 2015, 22 (8): 5908- 5918.
68 成玉婷, 吴小莲, 向垒, 等.. 广州市典型有机蔬菜基地土壤中磺胺类抗生素污染特征及风险评价. 中国环境科学, 2017, 37 (3): 1154- 1161.
69 张姚姚. 污染场地中氟喹诺酮类抗生素的风险评价、污染行为及控制研究 [D]. 上海: 东华大学, 2017.
70 丁丹, 黄晓依, 顾静仪, 等.. 畜禽粪肥还田四环素类抗生素(TCs)在土壤-蔬菜系统的分布特征及风险评估. 环境科学, 2023, 44 (8): 4440- 4447.
Options
文章导航

/

〈 〉