干旱胁迫下甘蓝型油菜相关抗旱基因的表达分析

doi:10.3969/j.issn.10005641.2016.01.015

华东师范大学学报(自然科学版) ›› 2016, Vol. 2016 ›› Issue (1): 113-122.doi: 10.3969/j.issn.10005641.2016.01.015

干旱胁迫下甘蓝型油菜相关抗旱基因的表达分析

何巧丽，张喆，冉春燕，谢小玉

西南大学农学与生物科技学院三峡库区生态环境教育部重点实验室南方山地农业教育部工程研究中心，重庆400716

收稿日期:2014-12-10 出版日期:2016-01-25 发布日期:2016-03-10
通讯作者: 谢小玉，女，副教授，研究方向为植物生理与分子生物学. E-mail:xiexy8009@163.com.
作者简介:何巧丽，女，硕士研究生，研究方向为植物生理与分子生物学. Email: hql1211@126.com.
基金资助:
国家自然科学基金（31271673）;公益性行业(农业)科研专项201503127；重庆市自然科学基金（CSTC2010BB1012）

Expression analysis of Brassica napus L. droughtrelated genes under drought stress

HE Qiao-Li, ZHANG Zhe, RAN Chun-Yan, XIE Xiao-Yu

Received:2014-12-10 Online:2016-01-25 Published:2016-03-10

摘要/Abstract

摘要： 以抗旱性较强的甘蓝型油菜Holiday为材料，在开花初期对油菜进行干旱胁迫处理，采用RTqPCR技术分析ABA2、BnSOS2、BnCS、CAM、CBF4、PIP1这6个油菜抗旱相关基因在干旱胁迫第1天、3天、5天、7天在根、茎、叶、花和青荚中的表达量.结果表明，干旱胁迫下，6个抗旱相关基因在油菜的不同器官中均出现了上调表达；在不同干旱胁迫下，各基因表达量呈现不同的变化趋势；在相同的器官中，各基因的表达量存在明显的不同，累积表达量表现为根中ABA2最大、CBF4最小，茎中CAM最大、CBF4最小，叶中PIP1最大、ABA2最小，花中CBF4最大、BnSOS2最小，青荚中BnCS最大，CBF4最小.说明植物在受到干旱胁迫时，不同的抗旱途径对干旱胁迫的响应程度是不同的；不同器官中各抗旱相关基因与胁迫时间的相关性分析表明，CAM基因在茎中的表达量、CBF4基因在花中的表达量与胁迫时间呈显著正相关.

关键词: 甘蓝型油菜, 干旱胁迫, 抗旱基因, 基因表达, 实时定量PCR

Abstract: The high droughttolerant variety Holiday was treated as the experimental material under drought stress in the early flower stage. The expression level of 6 droughtrelated genes (ABA2、BnSOS2、BnCS、 CAM、CBF4、PIP1) which were selected from different organs (root, stem, leaf, flower and green pod) at different stress times (1,3,5,7 days) were analyzed by qRT PCR technology. The result showed that the expression of six droughtresistant genes in different organs all increased under drought stress, and they presented different trends respectively under different drought stress. In the same organs, the expression of the six droughtresistant genes had obvious different. In root, the maximum cumulative expression value gene was ABA2, and the minimum cumulative expression value gene was CBF4; in stem, the maximum was CAM, the minimum was CBF4; in leaf, the maximum was PIP1, the minimum was ABA2; in flower, the maximum was CBF4, the minimum was BnSOS2; in green pod, the maximum was BnCS, the minimum was CBF4. It suggested that different drought resistance pathway played〖JP2〗 different role under drought stress. The correlation analysis between expression values of droughtrelated genes and stress time showed that the expression quantity of CAM gene in the stem volume, CBF4 gene in the flowers was significantly positively related to the stress time.

Key words: Brassica napus L. (rapeseed), drought stress, drought related genes, gene expression, quantitative realtime PCR

中图分类号:

S565.4

何巧丽, 张喆, 冉春燕, 谢小玉. 干旱胁迫下甘蓝型油菜相关抗旱基因的表达分析[J]. 华东师范大学学报(自然科学版), 2016, 2016(1): 113-122.

HE Qiao-Li, ZHANG Zhe, RAN Chun-Yan, XIE Xiao-Yu. Expression analysis of Brassica napus L. droughtrelated genes under drought stress[J]. Journal of East China Normal University(Natural Sc, 2016, 2016(1): 113-122.

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干旱胁迫下甘蓝型油菜相关抗旱基因的表达分析

Expression analysis of Brassica napus L. droughtrelated genes under drought stress

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