华东师范大学学报(自然科学版) ›› 2021, Vol. 2021 ›› Issue (6): 81-87.doi: 10.3969/j.issn.1000-5641.2021.06.009

• 计算机科学 • 上一篇    下一篇

基于最佳拼接路径的大视野显微图像研究

许阳1, 刘洪英1,2,*(), 庄泉洁2   

  1. 1. 华东师范大学 通信与电子工程学院, 上海  200241
    2. 上海澜澈生物科技有限公司, 上海  200240
  • 收稿日期:2020-08-31 出版日期:2021-11-25 发布日期:2021-11-26
  • 通讯作者: 刘洪英 E-mail:hyliu@ee.ecnu.edu.cn
  • 基金资助:
    上海市闵行区科委2018年度产学研合作计划(2018MH306)

Research on large-field microscopic images based on the best stitching path

Yang XU1, Hongying LIU1,2,*(), Quanjie ZHUANG2   

  1. 1. School of Communication and Electronic Engineering, East China Normal University, Shanghai  200241, China
    2. Shanghai Lanche Biological Technology Co., Ltd., Shanghai  200240, China
  • Received:2020-08-31 Online:2021-11-25 Published:2021-11-26
  • Contact: Hongying LIU E-mail:hyliu@ee.ecnu.edu.cn

摘要:

图像拼接技术是大视野显微数字图像应用中的关键技术之一. 随着科学技术的发展, 人们更加关心大视野显微数字图像的快速而又准确的图像拼接问题. 而现有的传统图像拼接方法是在图像配准之后按照固定的顺序拼接, 这对显微数字图像的采集质量以及配准的准确度要求很高, 一旦有误差便会沿着固定的路径累加, 从而使后续的图像产生错位等问题. 通过实验分析, 提出了一种优化大视野图像拼接路径的方法, 极大地优化了误差累积和配准失败带来的问题, 有效地提高了大视野显微数字图像的拼接质量. 该方法不仅可用于大视野显微图像的拼接, 也适用于其他类型的图像拼接.

关键词: 大视野显微数字图像, 误差累积, 图像配准, 最佳拼接路径

Abstract:

Image stitching technology is one of the key technologies in the application of large-field microscopic digital images. The existing traditional image stitching method is to stitch in a fixed order after image registration, and once there is an error, it will be accumulated along a fixed path, thereby causing problems such as misalignment of subsequent images. In this study, through experimental analysis, a method for optimizing the stitching path of the large-field image was proposed, which greatly optimized the problems caused by error accumulation and registration failure, and effectively improved the stitching quality of the large-field microscopic digital image. This method can be used not only for the stitching of large-field microscopic images, but also for other types of stitching.

Key words: large-field microscopic digital image, error accumulation, image registration, optimal stitching path

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