华东师范大学学报(自然科学版) ›› 2003, Vol. 2003 ›› Issue (1): 98-102.

• 论文 • 上一篇    下一篇

葡萄籽原花青素提取物抗氧化作用研究

朱振勤1； 翟万银2； 陈季武1； 夏晶1； 傅蓓蓓1； 谢萍1； 胡天喜1   

    1. 华东师范大学 生命科学学院， 上海 200062；
    2. 中国科学院硅酸研究所 生物材料与组织工程中心，上海 200050
  • 收稿日期:2002-03-02 修回日期:2002-10-19 出版日期:2003-03-25 发布日期:2003-03-25

Study on the Antioxidation Effects of Grape Seed Procyanidins Extract

ZHU Zhen-qin1; ZHAI Wan-yin2; CHEN Ji-wu1; XIA Jing1; FU Bei-bei1; XIE Ping1; HU Tian-xi1   

    1. School of Life Science, East China Normal University, Shanghai 200062, China;
    2. Center for Bio-materials and Tissue Engineering, Shanghai Institute of Silicates, Chinese Academy of Science, Shanghai 200050, China
  • Received:2002-03-02 Revised:2002-10-19 Online:2003-03-25 Published:2003-03-25

摘要： 为研究葡萄籽原花青素提取物（grape procyanidins extract,GPE）抗氧化和对DNA氧化损伤的保护作用，我们采用七种生物化学发光体系，检测了GPE 对O2-、OH、H2O2、ONOO-和全血嗜中性白细胞“呼吸爆发”产生的多种活性氧的清除作用，以及对OH引起的DNA氧化损伤的保护作用；采用Fe2＋诱发脂蛋白多不饱和脂肪酸过氧化比色体系，检测了GPE对脂质过氧化分抑制作用。结果显示，GPE 能有效地清除O2-、OH、H2O2、ONOO-和全血嗜中性白细胞“呼吸爆发”产生的多种活性氧而抑制体系 发光，并有效抑制脂质过氧化，半抑制浓度分别约为0.2ug/ml、90ug/ml、0.5ug/ml、10ug/ml、130ug/ml、70ug/ml;25ug/ml的GPE对DNA氧化损伤的抑制率誉为70％。提示GPE 能有效清除多种自由基，保护DNA免受·OH引起的氧化损伤，嗜良好的抗氧化剂。

关键词: 葡萄籽原花青素, 活性氮, DNA氧化损伤, 抗氧化剂, 葡萄籽原花青素, 活性氮, DNA氧化损伤, 抗氧化剂

Abstract: In order to study the antioxidation effects of grape seed procyanidins extract (GPE), seven chemiluminescence systems were used to determine the protective effect of GPE on DNA damage caused by ·OH and the scavenging effect of GPE on O2-、OH、H2O2 and ONOO- , and various reactive oxygen species produced during "respiration burst" of whole blood phagocytes. The inhibiting effect of GPE on lipid peroxidation was evaluated by using the colorimetric system of lipoprotein polyunsaturated fatty acids peroxidation induced by Fe2＋. The experiments showed that GPE can effectively scavenge O2-、OH、H2O2 ,ONOO- and various reactive oxygen species prouduced during "respiration burst" of whole blood phagocytes, and inhibit lipid peroxidation (IC50s are 0.2ug/ml、90ug/ml、0.5ug/ml、10ug/ml、130ug/ml、70ug/ml respectively); the inhibiting rate of DNA oxidative damage is approximately 70%, when GPE at a concentration of 25ug/ml is given. These results indicate that GPE os an efficient antioxidant and can effectively scavenge various free radicals and provide effective protection anfinst DNA damage caused by ·OH.

Key words: reactive oxygen species, reactive nitrogen species, DNA oxidative damage, antioxidant, grape seed procyanidins, reactive oxygen species, reactive nitrogen species, DNA oxidative damage, antioxidant