戊二醛固定剂对海洋微微型浮游植物特性的影响

华东师范大学学报(自然科学版) ›› 2004, Vol. 2004 ›› Issue (3): 93-98.

戊二醛固定剂对海洋微微型浮游植物特性的影响

张利华¹，晁敏²

1. 华东师范大学河口海岸国家重点实验室,上海 200062；2. 中国水产研究院东海水产研究所,上海 200090

收稿日期:2002-11-30 修回日期:2003-01-14 出版日期:2004-09-25 发布日期:2004-09-25
通讯作者: 张利华

The lmpact of Glutaraldehyde as Fixative on Marine Picophytoplankton

ZHANG Li-hua¹, CHAO Min²

State Key Laboratory of Estuarine & Coastal Research, East China Normal University,Shanghai 200062,China

Received:2002-11-30 Revised:2003-01-14 Online:2004-09-25 Published:2004-09-25
Contact: ZHANG Li-hua

摘要/Abstract

摘要： 试验比较了新鲜样品和经1%戊二醛固定液氮保存的样品在细胞密度、细胞大小、自发荧光强度等参数变化，结果表明，经戊二醛固定处理后，3种微微型浮游植物（原绿球藻、聚球藻和微型真核浮游植物）的细胞密度都有不同程度的增加；原绿球藻和聚球藻的细胞大小有不同程度的增大，而微型真核浮游植物则减小；三者的自发荧光强度都减弱.

关键词: 流式细胞术, 戊二醛, 微微型浮游植物, 流式细胞术, 戊二醛, 微微型浮游植物

Abstract: Samples are fixed with 1% glutaraldehyde and stored in liquid Nitrogen .Cell density、size and intensity of autofluorescence are compared with fresh samples in this experiment . The results showed that cell density and cell size of all three picophytoplanktonic populations(prochlorococcus、 synechococcus and picoeukaryotes) increased, but intensity of autofluorescence decreased.

Key words: glutaraldehyde, picophytoplankton, flow cytometry, glutaraldehyde, picophytoplankton

中图分类号:

Q94-33

张利华;晁敏. 戊二醛固定剂对海洋微微型浮游植物特性的影响[J]. 华东师范大学学报(自然科学版), 2004, 2004(3): 93-98.

ZHANG Li-hua;CHAO Min. The lmpact of Glutaraldehyde as Fixative on Marine Picophytoplankton[J]. Journal of East China Normal University(Natural Sc, 2004, 2004(3): 93-98.