华东师范大学学报(自然科学版) ›› 2007, Vol. 2007 ›› Issue (2): 104-110.

• 生命科学 • 上一篇    下一篇

人CREB4转核机制的初步研究

刘永强， 倪晓华， 周丹， 王鑫， 郭伟伟   

  1. 华东师范大学 生命科学学院,上海 200062
  • 收稿日期:2006-01-06 修回日期:2006-05-24 出版日期:2007-03-25 发布日期:2007-03-25
  • 通讯作者: 倪晓华

Primary Study of CREB4 Translocation Mechanism from Cytoplasm to Nucleus(Chinese)

LIU Yong-qiang, NI Xiao-hua, ZHOU Dan, WANG Xin, GUO Wei-wei   

  1. School of Life Science, East China Normal University, Shanghai 200062, China
  • Received:2006-01-06 Revised:2006-05-24 Online:2007-03-25 Published:2007-03-25
  • Contact: NI Xiao-hua

摘要：

cAMP 反应元件结合蛋白（cAMP response element-binding proteins，CREB）是一个哺乳动物转录因子家族，通过cAMP 反应元件（cAMP response element，CRE）调节cAMP和钙离子依赖性基因的表达.CREB4是CREB转录因子家族的一员.经酵母双杂交筛选人胎脑文库发现CREB4215-279aa可能与核转运因子kayopherinα2相互作用，提示karyopherinα2可能参与CREB4的跨膜转运过程.亚细胞定位结果显示，CREB4全长定位于细胞质，而缺失C端假定转膜结构域的CREB41-279aa蛋白则转移至细胞核内.荧光共定位进一步显示，CREB4和karyopherinα2共定位于细胞质中，CREB41-279aa和karyopherinα2共定位于细胞核中.结果提示C端被切除之后，CREB4被karyopherinα2转运到核内发挥转录作用.

关键词: CREB4, 酵母双杂交, 荧光共定位, kayopherinα2, CREB4, 酵母双杂交, 荧光共定位, kayopherinα2

Abstract: cAMP response element-binding(CREB) proteins are a family of mammalian transcription activators that mediate cAMP and calcium-dependent gene expression through the cAMP response element (CRE). CREB4 is a novel member of the human CREB family. Yeast two-hybrid system revealed that karyopherinα2 may associate with CREB4215-279aa. Subcellular location of CREB4 and CREB41-279aa showed that the GFP-CREB4 fusion protein was localized in cytoplasm, whereas the fusion protein of GFP-CREB41-279aa was translocated in nucleus. Our experiments further showed that CREB4 and karyopherinα2 were co-located in cytoplasm,but CREB41-279aa and karyopherinα2 were co-located in nucleus. These results suggested that first C-terminal was cleaved and then CREB4was carried into nucleus by karyopherinα2 to activate gene transcription.

Key words: yeast two-hybrid, fluorescent co-location, karyopherinα2, CREB4, yeast two-hybrid, fluorescent co-location, karyopherinα2

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