βCaMKII过量表达损害小鼠海马齿状回区长时程抑制

华东师范大学学报(自然科学版) ›› 2012, Vol. 2012 ›› Issue (1): 54-62.

βCaMKII过量表达损害小鼠海马齿状回区长时程抑制

徐浩，王勃，段燕红，曹晓华

华东师范大学脑功能基因组学教育部重点实验室，上海市脑功能基因组学重点实验室，上海 200062

收稿日期:2011-02-01 修回日期:2011-05-01 出版日期:2012-01-25 发布日期:2012-01-26

βCaMKII overexpression impairs long-term depression in dentate gyrus of mice

XU Hao, WANG Bo, DUAN Yan-hong, CAO Xiao-hua

Key Laboratory of Brain Functional Genomics, Ministry of Education, Shanghai Key Laboratory of Brain Functional Genomics, East China Normal University, Shanghai 200062, China

Received:2011-02-01 Revised:2011-05-01 Online:2012-01-25 Published:2012-01-26

摘要/Abstract

摘要： 采用海马齿状回（dentate gyrus, DG）区域特异性过量表达βCaMKII的蛋白修饰转基因小鼠，通过离体电生理技术，研究了βCaMKII高表达对该区域突触可塑性的影响.与对照组相比，转基因小鼠海马齿状回双脉冲抑制反应（paired-pulse depression, PPD）和颗粒细胞的电压—电流曲线（voltage-current curve）没有发生变化，而该区域的长时程抑制（long-term depression, LTD）明显被减弱.实验结果提示，βCaMKII的过量表达不影响小鼠海马齿状回区的突触前递质释放能力和颗粒细胞的被动属性，但损害其长时程抑制.这为进一步研究βCaMKII在学习记忆和突触可塑性中的作用提供了电生理学依据.

关键词: β钙离子/钙调素依赖的蛋白激酶II, 海马齿状回, 长时程抑制

Abstract: This experiment was designed to investigate the effects of the βCaMKII on the long-term depression (LTD) in dentate gyrus（DG）by using the transgenic mice in which the overexpression of βCaMKII is restricted to the DG area. Compared to the control group, the paired-pulse depression curve and voltage-current response in DG of transgenic mice didn’t change, but the LTD was significantly reduced. These results suggest that overexpression of the βCaMKII may impair the long-term depression without effect on the presynaptic function and passive properties of granule cells in DG area.

Key words: βCaMKII, dentate gyrus, long-term depression

中图分类号:

Q189

徐浩，王勃，段燕红，曹晓华. βCaMKII过量表达损害小鼠海马齿状回区长时程抑制[J]. 华东师范大学学报(自然科学版), 2012, 2012(1): 54-62.

XU Hao, WANG Bo, DUAN Yan-hong, CAO Xiao-hua. βCaMKII overexpression impairs long-term depression in dentate gyrus of mice[J]. Journal of East China Normal University(Natural Sc, 2012, 2012(1): 54-62.

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