关键词: 尿囊酸酶, 定量分析, 定位观察, gp150

Abstract: Location and expression of allanoicase were observed in the wild type KAx-3 and mutant type AK127 cells during development by immunofluorescent technique and Western blot, respectively. The results showed that abundant of allanoicase existed near the cytomembrane at the aggregation stage. At the mound stage, the fluorescence of allanoicase in the peripheral cells of mound was stronger than other place cells. When they developed into slug stage, more allanoicase existed in prestalk cells than that in prespore cells. The fluorescence of allanoicase expressed strongly at the junction of prespore area and prestalk area at the fruiting body stage,these cells would differentiate into prestalk B cells. These data indicated that there may be some relationships between the expression of allanoicase and cell-type differentiation during Dictysotelium discoideum development. The results of western blot showed that the expression of allanoicase increased gradually in the development of KAx-3 cells. The expression amount of allanoicase was maximum when the cells developed nearly 18 h. But the expression of allanoicase in AK127 cells which gene of gp150 protein was knocked out was always at a low level. It shows that the expression of allanoicase would be affected in the absence of gp150. The results indicate that the expression of allanoicase has some relationships with the developmental time of the cells, and is also associated with the expression of gp150 gene.

Key words: allanoicase, quantitative analysis, orientation observation, gp150