华东师范大学学报(自然科学版) ›› 2016, Vol. 2016 ›› Issue (3): 102-114.doi: 2016.03.012

• 生命科学 • 上一篇    下一篇

细胞外ATP影响铜离子诱导烟草悬浮细胞的死亡和H2O2的产生

白晶月,冯汉青,管冬冬,孙坤,贾凌云,李巧峡,张晋平   

  1. 西北师范大学~生命科学学院, 兰州~730070
  • 收稿日期:2015-06-19 出版日期:2016-05-25 发布日期:2016-09-22
  • 通讯作者: 贾凌云, 男, 博士研究生, 实验师, 研究方向为细胞与分子生物学 E-mail:lingyunjia1982@126.com
  • 作者简介:白晶月, 女, 硕士研究生, 研究方向为逆境植物生理生态.
  • 基金资助:

    国家自然科学基金(31260059, 30900105);
    教育部科学技术研究重点项目(211190);
    甘肃省财政厅高校基本科研业务项目;
    甘肃科技计划(145RJYA258)

Extracellular ATP affects the copper–induced cell death and H2O2 production in tobacco (Nicotiana tabacum L) cell-suspension cultures

BAI  Jing-Yue, FENG  Han-Qing, GUAN  Dong-Dong, SUN  Kun, JIA  Ling-Yun, LI  Qiao-Xia, ZHANG  Jin-Ping   

  • Received:2015-06-19 Online:2016-05-25 Published:2016-09-22

摘要: 细胞外ATP是植物体中许多生理过程的调节信号.胞外ATP可以影响铜离子诱导的细胞死亡和H_{2}O_{2}的产生.100sim 700 textit{mu }molL CuCl_{2}导致烟草悬浮细胞的死亡量显著上升,而且胞内H_{2}O_{2}和胞外H_{2}O_{2}的含量也随之上升.选择了300 textit{mu }molLCuCl_{2}研究铜离子导致的细胞死亡量上升和H_{2}O_{2}产生的过程.结果表明, 此浓度的 CuCl_{2}提升了NADPH氧化酶的活性,而加入DPI(NADPH氧化酶抑制剂)缓解了CuCl_{2}引起的细胞死亡和H_{2}O_{2}的产生,这说明CuCl_{2}引起细胞死亡和H_{2}O_{2}产生与NADPH氧化酶活性的增加相关. 加入50textit{mu }molLATP进一步增加了CuCl_{2}引起的细胞死亡、H_{2}O_{2}的产生、NADPH氧化酶.而DPI预处理后,外源ATP并未引起变化. 这一实验表明,胞外ATP可以通过刺激NADPH氧化酶影响铜离子引起的细胞活性和H_{2}O_{2}产生的变化

关键词: 细胞死亡, 铜离子, 细胞外ATP, H_{2}O_{2};NADPH氧化酶

Abstract: Extracellular ATP (eATP) has been considered as a mediating signal in several physiological processes of plants. In this article we showed that eATP can affect the copper-induced cell death and hydrogen peroxide (H_{2}O_{2}) production. CuCl_{2} at concentrations from 100 to 700 textit{mu }molL caused a
significant increase of cell death in tobacco (textit{Nicotiana tabacum} L) suspension cultures, and this increase of cell death level was followed with increases of both intracellular and extracellular H_{2}O_{2} production. The cells exposed to 300 textit{mu }molL CuCl_{2} were chosen to investigate the mechanisms for the copper-induced increases of cell death and H_{2}O_{2} production and the effect on this process. The results showed that the treatment_{ }with CuCl_{2 } at this concentration increased the activity of NADPH oxidase, and addition of DPI (diphenylene iodonium, an inhibitor of NADPH oxidase)alleviated the CuCl_{2}-induced increases of cell death and H_{2}O_{2} production, indicating that the CuCl_{2}-induced increases of cell death and H_{2}O_{2} production were related to an increase of the activity of NADPH oxidase. Addition of exogenous ATP at 50 textit{mu }molL into the CuCl_{2}-stressed cells further enhanced the levels of cell death,H_{2}O_{2} production, and NADPH oxidase activity. However, in the presence of DPI, exogenous ATP failed to do so. These observations indicated that eATP can affect the copper-induced changes of cell viability and H_{2}O_{2} production bystimulating NADPH oxidase

Key words: cell death;Cu^{2+ }ions;quad extracellular ATP, H_{2}O_{2;

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