华东师范大学学报(自然科学版) ›› 2004, Vol. 2004 ›› Issue (4): 103-110.

• 生命科学 • 上一篇    下一篇

盘基网柄菌(Dictyostelium discoideum ) KAX-3和AK127细胞形态发生和同工酶的比较研究

侯连生，马宁莎，王明，韩轶，付卓敏   

  1. 华东师范大学生命科学学院，上海200062
  • 收稿日期:2003-10-28 修回日期:2003-12-24 出版日期:2004-12-25 发布日期:2004-12-25
  • 通讯作者: 侯连生

Comparative Investigation of Morphogenesis and Isoenzymes between the Wild Cells KAX-3 and the Mutant Cells AK127 during the Dictyostelium Discoideum Development

HOU Lian-sheng, MA Ning-sha, WANG Yue, HAN Yi，FU Zhuo-ming   

  1. School of Life Science,East China Normal University, Shanghai 200062,China
  • Received:2003-10-28 Revised:2003-12-24 Online:2004-12-25 Published:2004-12-25
  • Contact: HOU Lian-sheng

摘要： 比较了盘基网柄菌(Dictyostelium discoideum)野生型细胞KAx-3和突变型细胞AK127在多细胞发育过程中的形态发生.两者有明显的区别;突变细胞的发育只能停留在细胞疏松聚集阶段，而野生型细胞能顺利完成整个发育过程.利用SDS-PAGE电泳比较分析了两者在重要发育阶段的三种同工酶:醋酶(EST)、苹果酸脱氢酶(MDH)和谷氨酸脱氢酶(GDH).结果发现AK127细胞发育阶段含有两种醋酶成分a和p，而KAX-3细胞中只有一种醋酶a;而且AK127细胞中苹果酸脱氢酶(MDH)含量在发育后14 h和16 h均高于KAX-3细胞;谷氨酸脱氢酶(GDH)同工酶在发育后14 h时相对含量高于KAX-3细胞，而在发育后16 h含量却低于KAX-3细胞.这些数据显示了两种类型细胞的同工酶有明显的差异;表明突变细胞基因的表达发生了一定的改变，由此说明了gp150分子在盘基网柄菌细胞发育中有重要作用.

关键词: 盘基网柄菌, 发育, 形态发生, SDS-PAGE, 谷氨酸脱氢酶(GDH), 苹果酸脱氢酶(MDH), 醋酶(EST), 盘基网柄菌, 发育, 形态发生, SDS-PAGE, 谷氨酸脱氢酶(GDH), 苹果酸脱氢酶(MDH), 醋酶(EST)

Abstract: Studying the morphogenesis of Dictyostelium discoideum development, we found that there were distinct differences between the wild cells KAX-3 and the mutant cells AK127(LagC gene was knockout). The mutant cells AK127 was blocked at loose aggregation stage for lacking gp150 during developmental stages. However, the wild cells KAX-3 completed the whole developmental stages successfully. Three isoenzymes were obtained from developing cells of Dictyostelium discoideum which included a wild type cells(KAX-3) and a mutant type cells Compared these isoenzymes by gel electrophoresis, we found that two different esterases appreared in AK127 while only one in KAX-3. Furthermore, the content of malate dehydrogenase (MDH) is more in AK127 than in KAX-3 at different developing stage. At 14h during development, the content of glutamate dehydrogenase (GDH) in AK127 is higher than in KAX-3;but at 16h GDH in AK127 is lower than in KAX-3. From the results, we learned that the pattern of some isoenzymes were changed between wild cells and mutant cells during development stage, indicating some changes in the expression of some genes. So it seemed that gp150 was an important factor in development.

Key words: development, morphogenesis, SDS-PAGE, Glutamate dehydrogenase(GDH), Malate dehydrogenase(MDH), Estersase(Est), dictyostelium, development, morphogenesis, SDS-PAGE, Glutamate dehydrogenase(GDH), Malate dehydrogenase(MDH), Estersase(Est)

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