白蚁肠道木质素分解菌PY12的分离鉴定及lip基因的克隆

华东师范大学学报(自然科学版) ›› 2014, Vol. 2014 ›› Issue (4): 113-121, 179.

白蚁肠道木质素分解菌PY12的分离鉴定及lip基因的克隆

高云航^1,2, 王巍¹, 刘萌¹, 勾长龙¹, 娄玉杰¹, 马红霞¹

1. 吉林农业大学~~动物科技学院, 长春\; 130118;
2. 吉林农业大学~~动物生产与产品质量安全教育部重点实验室,~~长春\; 130118

收稿日期:2013-10-01 修回日期:2014-01-01 出版日期:2014-07-25 发布日期:2014-07-25

Isolation and identification of lignin decomposing bacteria and cloning of lip gene in termite gut

GAO Yun-hang^1,2, WANG Wei¹, LIU Meng¹, GOU Chang-long¹, LOU YU-jie¹, MA Hong-xia¹

1. College of Animal Science and Technology,\ Jilin Agricultural University, Changchun} 130118, China; 2. Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Changchun 130118, China

Received:2013-10-01 Revised:2014-01-01 Online:2014-07-25 Published:2014-07-25

摘要/Abstract

摘要： 利用苯胺蓝平板法从白蚁肠道中分离到一株木质素分解高效菌株~PY~12,
经形态观察、生化鉴定和~16~S
rRNA~鉴定为肠杆菌属的霍氏肠杆菌~(\textit{Enterobacter hormaechei}).
根据已报道的~$lip$~(木质素过氧化物酶, Lignin
Peroxidase)~基因序列设计特异性引物, 克隆该菌的~$lip$~基因,
将其克隆入~pMD19-T~载体, 获得的核苷酸序列为~918~bp,
并与~GenBank~中已知的~$lip$~序列进行同源性对比, 同源性不高,
但该核苷酸翻译后的氨基酸序列比对结果发现,
其与假单胞杆菌~\textit{Pseudomonas} sp.
编码蛋白质的氨基酸序列同源性为88.5{\%}.

关键词: 白蚁, 木质素分解菌, 16S rRNA, 木质素过氧化物酶基因

Abstract: A strain of bacterium that named PY12 was isolated from
the termite gut using Azure B method, which had an efficiently
lignin decomposition function. It was identificated as
\textit{Enterobacter hormaechei}. by morphological, biochemical
identification and\\ 16S rRNA identification. The $lip$ gene were
amplified by the specific primers, and then cloning it into pMD19-T
vector, the target fragment sequences were 918 bp, Homology
comparison with the $lip$ gene which known in GenBank, the Homology
was low, but when the nucleotide sequences were translated into
protein, the homology was up to 88.5{\%} with the Pseudomonas sp..

Key words: Termite, lignin decomposing bacteria, 16S rRNA, lignin peroxidase gene

中图分类号:

S182

高云航, 王巍, 刘萌, 勾长龙, 娄玉杰, 马红霞. 白蚁肠道木质素分解菌PY12的分离鉴定及lip基因的克隆[J]. 华东师范大学学报(自然科学版), 2014, 2014(4): 113-121, 179.

GAO Yun-hang, WANG Wei, LIU Meng, GOU Chang-long, LOU YU-jie, MA Hong-xia. Isolation and identification of lignin decomposing bacteria and cloning of lip gene in termite gut[J]. Journal of East China Normal University(Natural Sc, 2014, 2014(4): 113-121, 179.

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