华东师范大学学报(自然科学版) ›› 2004, Vol. 2004 ›› Issue (4): 97-102.

• 生命科学 • 上一篇    下一篇

包囊游仆虫营养细胞和休眠细胞的胞器酶细胞化学

倪兵, 隋淑光, 顾福康   

  1. 华东师范大学生命科学学院,上海200062
  • 收稿日期:2003-09-24 修回日期:2003-10-28 出版日期:2004-12-25 发布日期:2004-12-25
  • 通讯作者: 倪兵

Studies on Electron Microscopic Enzymo-Cytochemistry of Organelles of Vegetative- and Resting-cell in Euplotes encysticus

Ni Bing, Sui Shuguang, Gu Fukang   

  1. Department of Biology, East China Normal University, Shanghai 200062
  • Received:2003-09-24 Revised:2003-10-28 Online:2004-12-25 Published:2004-12-25
  • Contact: Ni Bing

摘要： 为探索毛基体非吸收型休眠包囊的生命活动特征及其胞器的功能,本文应用电镜酶细胞化学方法检测了包囊游仆虫在不同生理状态下的胞器酶反应活性及其定位：（1）在营养细胞中的食物泡膜位置出现紧密排列的腺苷三磷酸酶反应颗粒,但在休眠细胞中酶反应颗粒则聚集在细胞表膜区；（2）营养期细胞中,酸性磷酸酶反应颗粒分布在处于不同消化时期的食物泡或自噬泡内,在休眠期细胞中酶反应颗粒定位在自噬泡周缘；（3）营养细胞的线粒体内膜有稀疏的琥珀酸脱氢酶反应颗粒,休眠细胞中在分散的线粒体内也有稀疏的酶反应颗粒,但反应活性比前者要弱得多.作者由所得结果推测,大部分情况下休眠细胞的酶反应活性较营养细胞的同种反应弱；不同类休眠细胞的生命活动中,其酶的功能和作用部位可能是不一样的；在休眠细胞中也发生着消化和营养利用的过程,也经历着线粒体的功能作用过程.

关键词: 包囊游仆虫, 休眠包囊, 酸性磷酸酶, 腺苷三磷酸酶, 琥珀酸脱氢酶, 包囊游仆虫, 休眠包囊, 酸性磷酸酶, 腺苷三磷酸酶, 琥珀酸脱氢酶

Abstract: The electron microscopic enzymo-cytochemistry is used to study on the organelles in both vegetative- and resting cells of Euplotes encysticus. The results revealed that: (1)The reaction particles of adenosine triposphatase (ATPase) was tightly confined to the membrane of food vacuoles in the vegetative cells, while in the resting cells they gathered around the pellicle. (2)In the vegetative cells, the localization of the reaction particles of acid phosphatase (ACPase) was in the food vacuoles or the autophagic vacuoles of different stages, while in the resting they formed the vacuolar or spherical bodies. (3)The reaction particles of succinate dehydrogenase scattered on the mitochondrial in the vegetative cells, though they could also be seen in the scattered mitochondria in the resting cells, its activity was weaker than that of the former. Thus it was supposed from the above results: (1) In most cases, the enzyme activities in the resting cells were weaker than in the vegetative cells. (2) The enzyme activity and localization might be different in different kinds of resting cells. (3) The resting cells could also have digestive duration, energy consumption and functional mitochondria.

Key words: Resting cyst, Electron microscopic, enzymo-cytochemistry, Euplotes encysticus, Resting cyst, Electron microscopic, enzymo-cytochemistry

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