华东师范大学学报(自然科学版) ›› 2005, Vol. 2005 ›› Issue (2): 58-64.

• 物理学 电子科学技术 • 上一篇    下一篇

脉冲电场对悬浮中国红豆杉细胞的影响

叶红1，黄琳玲1，陈树德1，钟建江2

  

  1. 1.华东师范大学 光谱学与波谱学教育部重点实验室，华东师范大学 物理系，上海200062；2.华东理工大学 生物反应器工程国家重点实验室，上海200237
  • 收稿日期:2003-11-28 修回日期:2004-01-13 出版日期:2005-05-25 发布日期:2005-05-25
  • 通讯作者: 叶红

Effects of Pulsed Electric Field on Suspension Cultures ofTaxus chinensis (Chinese)

YE Hong 1,HUANG Lin-ling1,CHEN Shu-de1,ZHONG Jian-jiang2   

  1. 1.Key Laboratory of optical and magnetic resonance spectroscopy, Minisitry of Education,Department of Physics, East China Normal University, Shanghai 200062,China;2.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai200237,China
  • Received:2003-11-28 Revised:2004-01-13 Online:2005-05-25 Published:2005-05-25
  • Contact: YE Hong

摘要： 以中国红豆杉悬浮细胞为植物细胞的模型，研究了低频脉冲电场对植物细胞生长和次生代谢的影响.不同生长时相的红豆杉细胞在不同时间的脉冲电场作用下，其生长和紫杉烷积累的情况表现出明显差异.对数前期的细胞经30 min的电场诱导后，细胞生长没有明显变化，但紫杉烷的含量提高了近30%.并发现， 脉冲电场和补糖的组合策略能更有效地促进细胞积累紫杉烷.

关键词: 中国红豆杉, 云南紫杉烷, 脉冲电场, 植物细胞培养, 中国红豆杉, 云南紫杉烷, 脉冲电场, 植物细胞培养

Abstract:

Suspension culture of Taxus chinensis was used as a model plant cell system to study the effects of pulsed electric field (PEF) on cell growth and the biosynthesis of secondary metabolites. Different exposure time of PEF had different effects on Taxus chinensis cells in different cell growth phases. Significant intracellular enhanced accumulation of taxuyunnanine C (Tc, a bioactive secondary metabolite) was observed by exposing the cells in early exponential growth phase to a 30 min PEF. The Tc content increased by 30% after exposure to PEF, without any loss of dry cell weight compared with that of the control. Moreover, combination of PEF with sucrose feeding showed a great synergistic accumulative effect on secondary metabolite production.

Key words: Taxuyunnanine C, pulsed electric field, plant cell culture, Taxus chinensis, Taxuyunnanine C, pulsed electric field, plant cell culture

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