工业废水中酚类污染物的毛细管电泳—安培检测应用研究

摘要/Abstract

摘要： 发展了毛细管电泳—安培检测方法，并将这一联用技术应用于苯酚、2,4-二氯苯酚、对硝基苯酚和邻、间、对甲酚的同时分离检测中.考察了氧化还原电位、缓冲溶液酸度、盐度、分离驱动电压及进样时间等因素对分析检测的影响.在优化实验条件下，以Na₂HPO₄-NaOH（pH 11.38）为缓冲体系，6种酚类物质能够在25 min内实现基线分离，氧化还原电位0.78 V (versus SCE) 下可以定量检测（三电极体系为：直径为300 μm的碳圆盘电极、饱和甘汞电极及铂电极）.实验结果表明，其线性达3个数量级(S/N=3)，检测限达10^-7mol/L. 本文还尝试把该方法应用于两个实际工业污水的酚类污染物的检测，其回收率为94.0%～107.0%，结果令人满意.因此，该方法可为政府及企业环境检测部门提供一种快速、准确、低廉、无污染、重现性高的质量控制方法.

关键词: 毛细管电泳, 分离, 安培检测, 污水, 酚类污染物

Abstract: A method based on capillary electrophoresis coupled with amperometric detection (CE-AD) was developed for the simultaneous determination of phenols: 2,4-dichlorophenol, p-nitrophenol, o-cresol, m-cresol and p-cresol. The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CE-AD were investigated. Under the optimum conditions, the aim analytes could be separated in buffer solution of Na₂HPO₄-NaOH at pH 11.38 within 25 min. A 300 μm diameter carbon disk electrode generated good current responses at +0.78 V ( vs SCE) for all analytes. The current responses were linear with concentrations over three orders of magnitude with detection limits at 10^-7mol/L (S/N=3) and the recoveries were in the range of 94.0%～107.0%. This method could be successfully applied to the analysis of actual sample from coking plants with satisfactory results.

Key words: capillary electrophoresis, separation, amperometric detection, wastewater, phenolic compounds

中图分类号:

X132

李自成，唐菀融，朱金坤，王清江，何品刚，方禹之. 工业废水中酚类污染物的毛细管电泳—安培检测应用研究[J]. 华东师范大学学报(自然科学版), 2013, 2013(1): 128-138.

LI Zi-cheng, TANG Wan-rong, ZHU Jin-kun, WANG Qing-jiang, HE Pin-gang, FANG Yu-zhi. Simultaneous determination of phenolic pollutants in wastewater using CE-AD[J]. Journal of East China Normal University(Natural Sc, 2013, 2013(1): 128-138.

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