华东师范大学学报(自然科学版) ›› 2015, Vol. 2015 ›› Issue (2): 84-90.doi: 10.3969/j.issn.1000-5641.2015.02.010

• “水体污染控制与治理”国家科技重大专项 • 上一篇    下一篇

黑臭河道底泥细菌群落结构对人工曝气响应的DGGE分析

何岩1,2，姚丽平1,2，李文超1,2，黄民生1,2，张一璠1,2   

  1. 1.  华东师范大学 上海市城市化生态过程与生态恢复重点实验室, 上海200241；
    2. 华东师范大学 生态与环境科学学院, 上海200241
  • 收稿日期:2014-08-07 出版日期:2015-03-25 发布日期:2015-05-28
  • 通讯作者: 黄民生，男，教授，博士生导师，主要研究方向为水环境治理与修复技术、废水处理工艺与技术、环境微生物及应用技术. E-mail:mshuang@des.ecnu.edu.cn
  • 作者简介:何岩，女，副教授，博士，研究方向为水环境治理与修复.E-mail: yhe@des.ecnu.edu.cn.
  • 基金资助:

    国家自然科学基金(41101471、51278192、41001347);上海市科委社会发展重点项目(12231201900); 中央高校基本科研业务费专项资金资助;国家科技重大专项（2013ZX07310001、2014ZX07101012

Responses of bacterial community structure in malodorous river sediments to aeration by DGGE

HE Yan 1,2,YAO Li-ping1,2,LI Wen-chao1,2,HUANG Min-sheng1,2,ZHANG Yi-fan1,2   

  1. 1. Shanghai Key Lab for Urban Ecological Processes and EcoRestoration, East China Normal University, Shanghai200241, China; 
    2. School of Ecological and Environmental Sciences, East China Normal University, Shanghai200241, China
  • Received:2014-08-07 Online:2015-03-25 Published:2015-05-28

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摘要： 采用基于16S rDNA的PCR DGGE (变性梯度凝胶电泳) 图谱并通过条带割胶回收DNA 进行序列分析，初步探讨了不同曝气条件下黑臭河道底泥中细菌群落结构多样性及其变化，同时用冗余分析（RDA）研究了环境因子与细菌群落结构之间的关系. 结果表明，人工曝气对黑臭河道底泥细菌群落结构产生明显的影响，并且随不同曝气强度底泥细菌群落多样性呈现不同的变化，其中当曝气扰动雷诺数（Re）为1810，溶解氧（DO）为7.35时，细菌优势群落多样性最高；序列比对分析推测适度的曝气有利于促进碳、氮、硫循环相关细菌的生长，其中以变形菌门为主导；冗余分析显示DO和Re对细菌群落结构影响显著.

关键词: 黑臭河道, 底泥, 曝气, 细菌, DGGE

Abstract: In this study, 16S rDNA based PCR amplification and denaturing gradientgel electrophoresis (PCRDGGE) as well as sequence alignment from the excised DGGE bands were used to investigate the bacterial diversity and variation. In addition, the redundancy analysis was adopted to assess the relationship between bacterial community structure and environmental factors. The results showed that aeration had marked effects on bacterial community structure in malodorous river sediments and different aeration intensities resulted in variable bacterial community. Amongst them the highest richness of bacterial community was observed with the aeration turbulence of Re=1 810 and DO of 7.35. It was also inferred that moderate aeration facilitated the growths of related bacteria to N and Scycling and theProteobacteria was dominant. It was also found that DO and Re played important roles in bacterial community from river sediments.

Key words: malodorous river, sediments, aeration, bacteria, DGGE

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