中国河口沉积物抗生素及抗性基因地理格局和驱动机制

doi:10.3969/j.issn.1000-5641.2024.06.008

华东师范大学学报（自然科学版） ›› 2024, Vol. 2024 ›› Issue (6): 86-98.doi: 10.3969/j.issn.1000-5641.2024.06.008

中国河口沉积物抗生素及抗性基因地理格局和驱动机制

尹国宇¹(), 郑栋升², 李晔¹, 黄晔¹, 刘敏¹^,*()

1. 华东师范大学地理科学学院地理信息科学教育部重点实验室, 上海　200241
2. 清华大学地球系统科学系, 北京　100084

收稿日期:2024-07-29 接受日期:2024-07-29 出版日期:2024-11-25 发布日期:2024-11-29
通讯作者: 刘敏 E-mail:gyyin@geo.ecnu.edu.cn;mliu@geo.ecnu.edu.cn
作者简介:尹国宇, 男, 副教授, 研究方向为河口生物地球化学. E-mail: gyyin@geo.ecnu.edu.cn
基金资助:
国家重点研发计划青年科学家项目 (2022YFC3105800); 国家自然科学基金 (42230505, 42206148,42476153)

Geographical pattern and driving mechanism of antibiotics and antibiotic resistance genes in estuarine sediment of China

Guoyu YIN¹(), Dongsheng ZHENG², Ye LI¹, Ye HUANG¹, Min LIU¹^,*()

1. Key Laboratory of Geographic Information Science (Ministry of Education), School of Geographic Sciences, East China Normal University, Shanghai　200241, China
2. Department of Earth System Science, Tsinghua University, Beijing　100084, China

Received:2024-07-29 Accepted:2024-07-29 Online:2024-11-25 Published:2024-11-29
Contact: Min LIU E-mail:gyyin@geo.ecnu.edu.cn;mliu@geo.ecnu.edu.cn

摘要/Abstract

摘要：

河口是抗生素抗性基因(antibiotic resistance genes, ARGs)的重要汇集区域, 但对于河口环境中ARGs的地理格局和主要驱动因素了解不足. 为此, 本研究通过高通量定量聚合酶链反应(high-throughput quantitative polymerase chain reaction, HT-qPCR)技术研究了中国16个典型河口在干湿季节的ARGs赋存特征, 并结合统计分析揭示了多种因素对ARGs的影响. 结果表明, 干季ARGs丰度高于湿季, 且ARGs丰度随纬度升高呈增加趋势. 人类活动、可移动基因元件、肠道微生物、抗生素浓度、理化性质和气候变量对ARGs的赋存有显著影响. 其中, 气候变量和人类活动对ARGs影响最显著. 影响ARGs的最重要气候变量是温度, 温度升高使得ARGs丰度降低. 研究证实, 人类活动和气候因素共同驱动了河口ARGs的丰度变化, 可为未来气候变化和社会经济发展背景下ARGs的控制政策提供依据.

关键词: 抗生素, 抗生素抗性基因, 河口沉积物, 人类活动, 气候变量

Abstract:

Estuaries are important convergence areas of antibiotic resistance genes (ARGs), but the geographical pattern and main drivers of ARGs in estuarine environments are poorly understood. Therefore, high-throughput quantitative polymerase chain reaction (HT-qPCR) was used to study the prevalence of ARGs in 16 estuaries of China in dry and wet seasons, and statistical analysis was utilized to reveal the influence of various factors on the prevalence of ARGs. The results showed that the abundance of ARGs was higher in the dry season than the wet season, and the abundance of ARGs increased with latitude. Human activities, mobile genetic elements, gut microbes, antibiotic concentrations, physicochemical properties, and climate variables had significant effects on the prevalence of ARGs. Climate variables and human activities had the most significant influence on ARGs. The most important climate variable affecting ARGs was temperature, with an increasing temperature reducing the abundance of ARGs. This study confirmed that human activities and climate factors jointly drive the changes in ARG abundance in estuaries. These findings provide a basis for the development of control policies for ARGs in the context of climate change and socio-economic development in the future.

Key words: antibiotics, antibiotic resistance genes, estuarine sediment, human activities, climate variables

中图分类号:

尹国宇, 郑栋升, 李晔, 黄晔, 刘敏. 中国河口沉积物抗生素及抗性基因地理格局和驱动机制[J]. 华东师范大学学报（自然科学版）, 2024, 2024(6): 86-98.

Guoyu YIN, Dongsheng ZHENG, Ye LI, Ye HUANG, Min LIU. Geographical pattern and driving mechanism of antibiotics and antibiotic resistance genes in estuarine sediment of China[J]. Journal of East China Normal University(Natural Science), 2024, 2024(6): 86-98.

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参考文献 51

1	NADIMPALLI M L, MARKS S J, MONTEALEGRE M C, et al.. Urban informal settlements as hotspots of antimicrobial resistance and the need to curb environmental transmission. Nature Microbiology, 2020, (5): 787- 795.
2	ZHENG D, YIN G, LIU M, et al.. Global biogeography and projection of soil antibiotic resistance genes. Science Advances, 2022, 8 (46): abq8015.
3	O’NEILL J. Antimicrobial Resistance: Tackling a Crisis for the Health and Wealth of Nations [M]. London: Review on Antimicrobial Resistance, 2014: 1-16.
4	DCOSTA V M, KING C E, KALAN L, et al.. Antibiotic resistance is ancient. Nature, 2011, 477, 457- 461.
5	BAKKEREN E, DIARD M, HARDT W D.. Evolutionary causes and consequences of bacterial antibiotic persistence. Nature Reviews Microbiology, 2020, 18, 479- 490.
6	KLEIN E Y, VAN BOECKEL T P, MARTINEZ E M, et al.. Global increase and geographic convergence in antibiotic consumption between 2000 and 2015. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115, E3463- E3470.
7	VAN BOECKEL T P, PIRES J, SILVESTER R, et al.. Global trends in antimicrobial resistance in animals in low- and middle-income countries. Science, 2019, 365, 6459.
8	HENDRIKSEN R S, MUNK P, NJAGE P, et al.. Global monitoring of antimicrobial resistance based on metagenomics analyses of urban sewage. Nature Communications, 2019, (10): 1- 12.
9	HAMIWE T, KOCK M M, MAGWIRA C A, et al.. Occurrence of enterococci harbouring clinically important antibiotic resistance genes in the aquatic environment in Gauteng, South Africa. Environmental Pollution, 2019, 245, 1041- 1049.
10	PÄRNÄNEN K M M, NARCISO-DA-ROCHA C, KNEIS D, et al.. Antibiotic resistance in European wastewater treatment plants mirrors the pattern of clinical antibiotic resistance prevalence. Science Advances, 2019, (5): eaau9124.
11	BAKKEREN E, HUISMAN J S, FATTINGER S A, et al.. Salmonella persisters promote the spread of antibiotic resistance plasmids in the gut. Nature, 2019, 573, 276- 280.
12	DELGADO-BAQUERIZO M, GUERRA C A, CANO-DÍAZ C, et al.. The proportion of soil-borne pathogens increases with warming at the global scale. Nature Climate Change, 2020, (10): 550- 554.
13	ZHU Y G, ZHAO Y, LI B, et al.. Continental-scale pollution of estuaries with antibiotic resistance genes. Nature Microbiology, 2017, (2): 1- 7.
14	ZHANG Q Q, YING G G, PAN C G, et al.. Comprehensive evaluation of antibiotics emission and fate in the river basins of China: Source analysis, multimedia modeling, and linkage to bacterial resistance. Environmental Science & Technology, 2015, 49 (11): 6772- 6782.
15	LYU M, LUAN X, LIAO C, et al.. Human impacts on polycyclic aromatic hydrocarbon distribution in Chinese intertidal zones. Nature Sustainability, 2020, 3 (10): 878- 884.
16	SUN D, TANG X, ZHAO M, et al.. Distribution and diversity of comammox nitrospira in coastal wetlands of China. Frontiers in Microbiology, 2020, 11, 589268.
17	GAO D, LI X, LIN X, et al.. Soil dissimilatory nitrate reduction processes in the Spartina alterniflora invasion chronosequences of a coastal wetland of southeastern China: Dynamics and environmental implications. Plant and Soil, 2017, (3): 383- 399.
18	HOU L, WANG R, YIN G, et al. Nitrogen fixation in the intertidal sediments of the Yangtze Estuary: Occurrence and environmental implications [J]. Journal of Geophysical Research Biogeosciences, 2018, 123: 936-944.
19	JIANG Y, YIN G, HOU L, et al. Variations of dissimilatory nitrate reduction processes along reclamation chronosequences in Chongming Island , China [J]. Soil & Tillage Research, 2021, 206(1): 104815.
20	YIN G, HOU L, ZONG H, et al.. Denitrification and anaerobic ammonium oxidization across the sediment–water interface in the hypereutrophic ecosystem, Jinpu Bay, in the Northeastern Coast of China. Estuaries and Coasts, 2015, 38, 211- 219.
21	SHI H, YANG Y, LIU M, et al.. Occurrence and distribution of antibiotics in the surface sediments of the Yangtze Estuary and nearby coastal areas. Marine Pollution Bulletin, 2014, 83 (1): 317- 323.
22	LIU L, SU J Q, GUO Y, et al.. Large-scale biogeographical patterns of bacterial antibiotic resistome in the waterbodies of China. Environment International, 2018, 117, 292- 299.
23	CHEN Q L, AN X L, LI H, et al.. Do manure-borne or indigenous soil microorganisms influence the spread of antibiotic resistance genes in manured soil?. Soil Biology and Biochemistry, 2017, 114, 229- 237.
24	AN X L, SU J Q, LI B, et al.. Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR. Environment International, 2018, 117, 146- 153.
25	SUN J, LIAO X P, D’SOUZA A W, et al.. Environmental remodeling of human gut microbiota and antibiotic resistome in livestock farms. Nature Communications, 2020, (11): 1- 11.
26	JIAO S, LIU Z, LIN Y, et al.. Bacterial communities in oil contaminated soils: Biogeography and co-occurrence patterns. Soil Biology and Biochemistry, 2016, 98, 64- 73.
27	WU L, NING D, ZHANG B, et al.. Author correction: Global diversity and biogeography of bacterial communities in wastewater treatment plants. Nature Microbiology, 2019, 4 (12): 2579.
28	DELGADO-BAQUERIZO M, MAESTRE F T, REICH P B, et al.. Microbial diversity drives multifunctionality in terrestrial ecosystems. Nature Communications, 2016, (7): 1- 8.
29	GUO X P, LIU X, NIU Z S, et al.. Seasonal and spatial distribution of antibiotic resistance genes in the sediments along the Yangtze Estuary, China. Environmental Pollution, 2018, 242, 576- 584.
30	LU X M, LU P Z.. Seasonal variations in antibiotic resistance genes in estuarine sediments and the driving mechanisms. Journal of Hazardous Materials, 2020, 383, 121164.
31	CHEN B, LIANG X, HUANG X, et al.. Differentiating anthropogenic impacts on ARGs in the Pearl River Estuary by using suitable gene indicators. Water Research, 2013, 47 (8): 2811- 2820.
32	LU J, ZHANG Y, WU J, et al.. Occurrence and spatial distribution of antibiotic resistance genes in the Bohai Sea and Yellow Sea areas, China. Environmental Pollution, 2019, 252, 450- 460.
33	LU J, ZHANG Y, WU J.. Continental-scale spatio-temporal distribution of antibiotic resistance genes in coastal waters along coastline of China. Chemosphere, 2020, 247, 125908.
34	ZHENG D, YIN G, LIU M, et al.. A systematic review of antibiotics and antibiotic resistance genes in estuarine and coastal environments. Science of the Total Environment, 2021, 777, 146009.
35	FRESIA P, ANTELO V, SALAZAR C, et al.. Urban metagenomics uncover antibiotic resistance reservoirs in coastal beach and sewage waters. Microbiome, 2019, (7): 1- 9.
36	CHEN C Q, ZHENG L, ZHOU J L, et al.. Persistence and risk of antibiotic residues and antibiotic resistance genes in major mariculture sites in Southeast China. Science of the Total Environment, 2017, 580, 1175- 1184.
37	GAO Q, LI Y, QI Z, et al.. Diverse and abundant antibiotic resistance genes from mariculture sites of China’s coastline. Science of the Total Environment, 2018, 630, 117- 125.
38	MUZIASARI W I, PÄRNÄNEN K, JOHNSON T A, et al.. Aquaculture changes the profile of antibiotic resistance and mobile genetic element associated genes in Baltic Sea sediments. FEMS Microbiology Ecology, 2016, 92, fiw052.
39	SU J Q, AN X L, LI B, et al.. Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China. Microbiome, 2017, (5): 1- 15.
40	GUO X, ZHAO S, CHEN Y, et al.. Antibiotic resistance genes in sediments of the Yangtze Estuary: From 2007 to 2019. Science of the Total Environment, 2020, 744, 140713.
41	PEHRSSON E C, TSUKAYAMA P, PATEL S, et al.. Interconnected microbiomes and resistomes in low-income human habitats. Nature, 2016, 533, 212- 216.
42	MACFADDEN D R, MCGOUGH S F, FISMAN D, et al.. Antibiotic resistance increases with local temperature. Nature Climate Change, 2018, (8): 510- 514.
43	ZHU D, MA J, LI G, et al.. Soil plastispheres as hotpots of antibiotic resistance genes and potential pathogens. The ISME Journal, 2022, 16 (2): 615.
44	BAHRAM M, HILDEBRAND F, FORSLUND S K, et al.. Structure and function of the global topsoil microbiome. Nature, 2018, 560, 233- 237.
45	DUNIVIN T K, SHADE A.. Community structure explains antibiotic resistance gene dynamics over a temperature gradient in soil. FEMS Microbiology Ecology, 2018, 94 (3): 1- 9.
46	SPANÒ S, GALÁN J E.. A Rab32-dependent pathway contributes to Salmonella typhi host restriction. Science, 2012, 338, 960- 963.
47	DU S, SHEN J P, HU H W, et al.. Large-scale patterns of soil antibiotic resistome in Chinese croplands. Science of the Total Environment, 2020, 712, 136418.
48	REVERTER M, SARTER S, CARUSO D, et al.. Aquaculture at the crossroads of global warming and antimicrobial resistance. Nature Communications, 2020, (11): 1- 8.
49	COLLIGNON P, BEGGS J J, WALSH T R, et al.. Anthropological and socioeconomic factors contributing to global antimicrobial resistance: A univariate and multivariable analysis. Lancet Planetary Health, 2018, (2): e398- e405.
50	王芳, 豆庆圆, 付玉豪, 等.. 土壤中有机肥源抗生素抗性基因环境归趋与风险管理研究进展. 农业环境科学学报, 2022, (12): 2563- 2576.
51	武晨, 黄凤莲, 刘新刚, 等.. 环洞庭湖土壤抗生素抗性基因分布和潜在风险. 中国环境科学, 2024, 44 (3): 1575- 1583.

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中国河口沉积物抗生素及抗性基因地理格局和驱动机制

Geographical pattern and driving mechanism of antibiotics and antibiotic resistance genes in estuarine sediment of China

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